Pluronic-encapsulated natural chlorophyll nanocomposites for in vivo cancer imaging and photothermal/photodynamic therapies
Pluronic-encapsulated natural chlorophyll nanocomposites for in vivo cancer imaging and photothermal/photodynamic therapies
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用于体内癌症成像和光热/光动力疗法的 Pluronic 封装的天然叶绿素纳米复合材料
DOI：
10.1016/j.biomaterials.2014.05.049
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发表时间：
2014-09-01
期刊：
影响因子：
14
通讯作者：
Shi, Donglu
中科院分区：
文献类型：
--
作者：
Chu, Maoquan;Li, Haikuo;Shi, Donglu
开发用于癌症诊断和治疗的纳米技术的一个巨大挑战是复杂临床程序所需的综合功能。在所有要求中，毒性一直是阻碍大多数纳米载体临床应用的主要障碍。本研究从蔬菜中提取叶绿素（Chl），并将其包封在聚合物（pluronic F68, Plu）胶束中，用于癌症成像和治疗。结果表明，含chl的纳米复合材料能够靶向小鼠肿瘤，并且在尾静脉注射后2天以上肿瘤部位内纳米复合材料的荧光仍保持高强度。有趣的是，口服纳米复合材料也成功地用于肿瘤靶成像。此外，还发现饲料中Chl能够有效地将近红外激光照射转化为热量。经纳米复合材料和辐照处理后，黑色素瘤细胞和小鼠肿瘤的生长均得到有效抑制。通过激光触发Chl的光热和光动力协同作用实现对肿瘤的抑制。Chl是一种来自蔬菜的天然物质，无毒，是癌症诊断和治疗的理想纳米载体。基于这项研究的结果，plus - chl纳米复合材料在未来的临床应用中显示出了希望。(C) 2014 Elsevier Ltd.版权所有。
A great challenge in developing nanotechnologies for cancer diagnosis and therapy has been the combined functionalities required for complicated clinical procedures. Among all requirements, toxicity has been the major hurdle that has prevented most of the nano-carriers from clinical use. Here, we extracted chlorophyll (Chl) from vegetable and encapsulated it into polymer (pluronic F68, Plu) micelles for cancer imaging and therapy. The results showed that the Chl-containing nanocomposites were capable of mouse tumor targeting, and the nanocomposite fluorescence within the tumor sites remained at high intensity more than two days after tail-vein injection. It is interesting that oral administration with the nanocomposites was also successful for tumor target imaging. Furthermore, the dietary Chl was found to be able to efficiently convert near-infrared laser irradiation to heat. The growths of melanoma cells and mouse tumors were effectively inhibited after being treated with the nanocomposites and irradiation. The suppression of the tumors was achieved by laser-triggered photothermal and photodynamic synergistic effects of Chl. As a natural substance from vegetable, Chl is non-toxic, making it an ideal nano-carrier for cancer diagnosis and treatment. Based on the results of this research, the Plu-Chl nanocomposites have shown promise for future clinical applications. (C) 2014 Elsevier Ltd. All rights reserved.