CHARACTERIZATION AND DISTRIBUTION OF NUCLEIC-ACID SEQUENCES OF A NOVEL TYPE-C RETROVIRUS ISOLATED FROM NEOPLASTIC HUMAN LYMPHOCYTES-T
CHARACTERIZATION AND DISTRIBUTION OF NUCLEIC-ACID SEQUENCES OF A NOVEL TYPE-C RETROVIRUS ISOLATED FROM NEOPLASTIC HUMAN LYMPHOCYTES-T
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DOI：
10.1073/pnas.78.3.1887
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
GALLO, RC
中科院分区：
文献类型：
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作者：
REITZ, MS;POIESZ, BJ;GALLO, RC
通过核酸杂交实验对一种C型逆转录病毒（被命名为HTLV[人类皮肤T细胞淋巴瘤病毒]）进行了特征分析，该病毒最近从一个源于皮肤T细胞淋巴瘤（蕈样真菌病）患者的淋巴结的细胞系中分离出来，后来又从其外周血中分离得到。HTLV的[³H]互补DNA与它自身的70S RNA有90%发生杂交，其动力学与其他逆转录病毒的遗传复杂性相符，但它与动物逆转录病毒（B型、C型和D型）的RNA或原病毒DNA基本不发生杂交，包括那些来自非人灵长类动物的病毒。其他逆转录病毒的[³H]互补DNA与产生HTLV的人类T细胞系的RNA或DNA不发生杂交，在这些细胞的DNA中存在HTLV原病毒序列（每个单倍体基因组2 - 3个拷贝），并且在细胞质RNA中存在同源序列（按重量计0.3%为病毒序列）。在来自其他各种人类组织的DNA中未发现与HTLV相关的核酸序列。HTLV显然是一种新型的C型病毒，它不是人类内源性（遗传性传播的）逆转录病毒。
A type C retrovirus (designated HTLV [human cutaneous T cell lymphoma virus]) recently isolated from a cell line derived from a lymph node and later from peripheral blood of a person with cutaneous T-cell lymphoma (mycosis fungoides) was characterized by nucleic acid hybridization experiments. HTLV [3H]c[complementary]DNA hybridized 90% to its own 70S RNA with kinetics consistent with the genetic complexity of other retroviruses, but it did not hybridize substantially to RNA or proviral DNA from animal retroviruses (types B, C and D), including those from nonhuman primates. [3H]cDNA from other retroviruses did not hybridize to RNA or DNA of the human T-cell line producing HTLV, HTLV proviral sequences were present (2-3 copies per haploid genome) in DNA of these cells, and homologous sequences were present in the cell cytoplasmic RNA (0.3% viral sequences by weight). HTLV-related nuclei acid sequences were not found in DNA from various other human tissues. HTLV is apparently is a new class of type C virus that is not an endogenous (genetically transmitted) retrovirus in man.