Molecular analysis of microflora associated with dentoalveolar abscesses
Molecular analysis of microflora associated with dentoalveolar abscesses
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DOI：
10.1128/jcm.34.3.537-542.1996
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发表时间：
1996-03-01
影响因子：
9.4
通讯作者：
Wade, WG
中科院分区：
文献类型：
--
作者：
Dymock, D;Weightman, AJ;Wade, WG
用细菌培养和分子生物学方法对3例牙槽脓肿患者的菌群进行了检测。利用保守的真细菌引物随机扩增16S rRNA基因，并进行克隆。对培养细菌的16S rRNA克隆和扩增基因进行限制性内切酶片段长度多态性分析，以检测可能的不可培养细菌。对代表五个主要未培养生物群的克隆进行了测序。其中2株鉴定为牙龈卟啉单胞菌和口腔普雷沃特氏菌，1株与微小消化链球菌关系密切。剩下的两个克隆并不对应于已知的、之前测序的生物。其中一种与需氧水生生物Zoogloea ramigera有亲缘关系，另一种与Prevotel属有较远的亲缘关系。这项研究证明了用分子方法表征与人类感染相关的微生物区系的可能性，而不存在培养的固有偏见。
The microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16S rRNA genes were randomly amplified by means of conserved eubacterial primers and cloned. Restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16S rRNA from the cultured bacteria was used to detect putative unculturable bacteria. Clones representative of five predominant groups of uncultured organisms were sequenced. Two were identified as Porphyromonas gingivalis and Prevotella oris, and one was found to be closely related to Peptostreptococcus micros. The remaining two clones did not correspond to known, previously sequenced organisms. One was related to Zoogloea ramigera, a species of aerobic waterborne organisms, while the other was distantly related to the genus Prevotella. This study has demonstrated the possibility of the characterization of microflora associated with human infection by molecular methods without the inherent biases of culture.