Tri-trophic interactions involving pest aphids, predatory 2-spot ladybirds and transgenic potatoes expressing snowdrop lectin for aphid resistance
Tri-trophic interactions involving pest aphids, predatory 2-spot ladybirds and transgenic potatoes expressing snowdrop lectin for aphid resistance
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DOI：
10.1023/a:1009659316170
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发表时间：
1999-01-01
影响因子：
3.1
通讯作者：
Gatehouse, JA
中科院分区：
文献类型：
--
作者：
Birch, ANE;Geoghegan, IE;Gatehouse, JA
为增强抗虫性而进行基因工程改造的转基因作物应与IPM的其他组成部分相容，以使抗虫性持久有效。一个实验性马铃薯品系被基因工程化以表达抗蚜虫植物蛋白（雪花莲凝集素，GNA），并评估昆虫抗性基因与有益害虫捕食者的可能相互作用。这些扩展的实验室研究是第一次证明涉及表达凝集素的转基因作物、目标害虫蚜虫和有益的食蚜性捕食者的不利的三营养相互作用。二斑瓢虫Adalia bipunctata [L.]用定殖在叶片中表达GNA的转基因马铃薯的桃-马铃薯蚜虫（Myzuspersicae Sulzer）喂养12天，瓢虫的繁殖力、卵的存活力和寿命在随后的2-3周内显著降低。没有观察到由于转基因植物引起的急性毒性，尽管雌性瓢虫的寿命减少了51%。转基因马铃薯中的桃蚜对瓢虫繁殖的不利影响，在瓢虫转基因豌豆蚜虫后被逆转。这些结果表明，转基因马铃薯品系中抗虫凝集素基因的表达可通过其食物链中的蚜虫对捕食性瓢虫造成不利影响。这些潜在的生态风险在实地条件下的重要性需要进一步评估。
Transgenic crops genetically engineered for enhanced insect resistance should be compatible with other components of IPM for the pest resistance to be durable and effective. An experimental potato line was genetically engineered to express an anti-aphid plant protein (snowdrop lectin, GNA), and assessed for possible interactions of the insect resistance gene with a beneficial pest predator These extended laboratory studies are the first to demonstrate adverse tri-trophic interactions involving a lectin-expressing transgenic crop, a target pest aphid and a beneficial aphidophagous predator. When adult 2-spot ladybirds (Adalia bipunctata [L.]) were fed for 12 days on peach-potato aphids (Myzus persicae Sulzer) colonising transgenic potatoes expressing GNA in leaves, ladybird fecundity, egg viability and longevity significantly decreased over the following 2-3 weeks. No acute toxicity due to the transgenic plants was observed, although female ladybird longevity was reduced by up to 51%. Adverse effects on ladybird reproduction, caused by eating peach-potato aphids from transgenic potatoes, were reversed after switching ladybirds to feeding on pea aphids from non-transgenic bean plants. These results demonstrate that expression of a lectin gene for insect resistance in a transgenic potato line can cause adverse effects to a predatory ladybird via aphids in its food chain. The significance of these potential ecological risks under field conditions need to be further evaluated.