EXPRESSION OF GABA RECEPTOR RHO-1 AND RHO-2 SUBUNITS IN THE RETINA AND BRAIN OF THE RAT
EXPRESSION OF GABA RECEPTOR RHO-1 AND RHO-2 SUBUNITS IN THE RETINA AND BRAIN OF THE RAT
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DOI：
10.1111/j.1460-9568.1995.tb01144.x
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发表时间：
1995-07-01
影响因子：
3.4
通讯作者：
BORMANN, J
中科院分区：
文献类型：
--
作者：
ENZ, R;BRANDSTATTER, JH;BORMANN, J
我们研究了GABA受体rho 1和rho 2亚单位在大鼠中枢神经系统的分布。大鼠rho 1和rho 2 cDNA片段的克隆显示，在蛋白质水平上与相应的人类序列的相似性为99%（rho 1）和88%（rho 2）。虽然人rho 2亚基没有被蛋白激酶C磷酸化的共有序列，但大鼠序列的细胞质环包含两个这样的位点。使用聚合酶链反应与逆转录总RNA（RT-PCR）从不同的脑组织中发现，转录的rho 1亚基是目前只在视网膜。rho 2 mRNA在所有脑区均被检测到，其中视网膜中的表达水平最高。视网膜切片的原位杂交显示，rho 1和rho 2转录本存在于内核层。RT-PCR和原位杂交的分离的视网膜细胞表明，这两个rho亚基存在于杆双极细胞。由于这些细胞表达荷包牡丹碱不敏感的GABA受体，我们的结果进一步支持了rho亚基是GABA（C）受体的一部分的观点。
We have investigated the distribution of GABA receptor rho 1 and rho 2 subunits in the rat central nervous system. Cloning of rat rho 1 and rho 2 cDNA fragments revealed similarities to the corresponding human sequences of 99% (rho 1) and 88% (rho 2) at the protein level. Whereas the human rho 2 subunit has no consensus sequence for phosphorylation by protein kinase C, the cytoplasmic loop of the rat sequence contains two such sites. Use of the polymerase chain reaction with reverse-transcribed total RNA (RT-PCR) from different brain tissues revealed that transcript for the rho 1 subunit was present in the retina only. The rho 2 mRNA was detected in all brain regions, with the highest level of expression in the retina. In situ hybridization of retinal sections revealed that rho 1 and rho 2 transcripts are present in the inner nuclear layer. RT-PCR and in situ hybridization of isolated retinal cells showed that both rho subunits are present in rod bipolar cells. Since these cells express bicuculline-insensitive GABA receptors, our results further support the idea that rho subunits are part of the GABA(C) receptor.