QTL Mapping and Marker Identification for Sex-Determining: Indicating XY Sex Determination System in the Swimming Crab (Portunus trituberculatus).
QTL Mapping and Marker Identification for Sex-Determining: Indicating XY Sex Determination System in the Swimming Crab (Portunus trituberculatus).
复制标题
用于性别决定的 QTL 作图和标记识别：指示梭子蟹 (Portunus triuberculatus) 中的 XY 性别决定系统。
DOI：
10.3389/fgene.2018.00337
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发表时间：
2018
影响因子：
3.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Lv J;Sun D;Huan P;Song L;Liu P;Li J
性别决定是一个重要的研究领域，在进化和发育生物学中一直有一个耐人寻味的方面。性别的数量性状基因座(QTL)定位将有助于阐明性别决定系统。本研究在高密度连锁图谱的基础上，对梭子蟹进行了性别QTL定位，首次定位到一个连锁群(Lg)上的高度显著的QTL(LG24，LOD>；14)。关联分析表明，QTL区域有20个标记与性别显著相关，其中雄性异交基因支持XY性别决定机制。通过分离扭曲分析，在家系水平上确定了两个性别特异标记，这两个标记被认为是与三瘤拟青霉性别关系最密切的。根据性别标记序列(Marker3840、Marker20320和Marker10494)，鉴定了3个潜在的与性别相关的基因，实时定量聚合酶链式反应结果表明，这些基因在男性精子发生或性别特征中起重要作用。我们的结果将有助于性别决定基因的精细定位，并阐明三瘤拟青霉的性别决定机制。
Sex determination is an important area of research, which has always had an intriguing aspect in evolutionary and developmental biology. Quantitative trait locus (QTL) mapping for sex will be helpful in clarifying the sex determination system. In this study, the sex QTL mapping of the swimming crab (Portunus trituberculatus) was performed based on a high-density linkage map, and a highly significant QTL specifically mapped on a single linkage group (LG) was firstly identified (LG24, LOD > 14). Twenty markers in the QTL region showed significant associations with sex by association analysis, of which heterogametic genotypes in males supported the XY sex determination mechanism. Two sex-specific markers at the family level were identified via segregation distortion analysis, which were known to be the most closely linked to the sex of P. trituberculatus. Based on sex marker sequences (Marker3840, Marker20320, and Marker10494), three potential sex-related genes were identified, and the quantitative real-time PCR results suggested that these genes were important in spermatogenesis or sex characteristics in males. Our results will contribute to the fine-mapping of sex-determining genes and clarify the sex determination mechanism of P. trituberculatus.