Preparation of Astragalus membranaceus lectin and evaluation of its biological function
Preparation of Astragalus membranaceus lectin and evaluation of its biological function
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DOI：
10.3892/br.2018.1132
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发表时间：
2018-10-01
影响因子：
2.3
通讯作者：
Feng, Ma-Li
中科院分区：
文献类型：
--
作者：
Bai, Chong-Zhi;Hao, Jian-Qing;Feng, Ma-Li
膜荚黄芪凝集素（Astragalus membranaceus lectin，AML）是从膜荚黄芪（Astragalus membranaceus）种子中分离得到的一种分子量为67 kDa的凝集素。黄芪种子经醋酸盐处理、硫酸铵沉淀、HiTrap SP XL离子柱和Superdex G25凝胶过滤层析纯化后，得到黄芪粗提物。AML含有16.4%的糖，类似于70%的极性氨基酸和类似于30%的疏水性氨基酸。AML对人和动物红细胞，特别是人O型血和兔红细胞具有凝集活性。它还表现出耐酸碱性和64 ℃以上的热变性。与人正常肝HL-7702细胞相比，不同浓度的AML（6.25、12.50、25.00和50.00 μ g/ml）对SGC-7901、HepG 2和H 22癌细胞株的生长有上级抑制作用，并对细菌有明显的抗菌作用，半数最大抑菌浓度为B。痢疾杆菌S. aureus和E.大肠杆菌的最低抑菌浓度分别为85.4、80.2和65.3 μ g/ml。
Astragalus membranaceus lectin (AML) was abstracted as a supposedly novel agglutinin of 67 kDa from the seeds of Astragalus membranaceus. The seeds of Astragalus membranaceus were treated with acetate, ammonium sulfate precipitation, and purified by HiTrap SP XL ion column and Superdex G25 gel filtration chromatography to obtain the AML. AML contained 16.4% sugar, similar to 70% polar amino acids and similar to 30% hydrophobic amino acids. The AML exhibited agglutination activity toward human and animal erythrocytes, particularly human blood type O and rabbit erythrocytes. It also exhibited acid/alkali resistance and thermal denaturation above 64 degrees C. Compared with human normal liver HL-7702 cells, different concentrations of AML (6.25, 12.50, 25.00 and 50.00 mu g/ml) exhibited superior inhibitory effects on the growth of SGC-7901, HepG2 and H22 carcinoma cell lines, and displayed marked antibacterial effects on bacteria; the half maximal inhibitory concentration for B. dysenteriae, S. aureus and E. coli were 85.4, 80.2 and 65.3 mu g/ml, respectively.