Population Dynamics in Antibody Producing CHO Cell Cultures
Population Dynamics in Antibody Producing CHO Cell Cultures
复制标题
产生抗体的 CHO 细胞培养物中的群体动态
DOI：
10.1002/cite.201855335
复制
发表时间：
2018
影响因子：
1.9
通讯作者：
Pörtner
中科院分区：
文献类型：
--
作者：
Möller;Pörtner
这张海报讨论了一个12周的克隆开发过程。该过程从硅开始，通过筛选和克隆选择在96深孔板到5l摇瓶中进行。机器学习和蛋白质工程使得生成大量高表达的稳定细胞系变得快速和容易。这样的细胞系正在推动生物制造从大规模生产向小型高效生产转变。为了从众多候选产品中选出最佳产品，克隆筛选、选择和扩展工具变得比以往任何时候都更加重要。高表达细胞系的现成可用性使得使用孵育摇床代替生物反应器来满足许多生物生产需求成为可能。孵育摇床已经是细胞系筛选和发育的标准配置。因此，它们提供了从克隆筛选一直到cGMP生产的简单且易于验证的路径。
This poster discusses a process for a 12-week clone development process. The process starts in silico and progresses through screening and clone selection in 96-deepwell plates up to 5L-shaker flasks. Machine learning and protein engineering make it fast and easy to generate large numbers of high expressing stable cell lines. Such cell lines are moving biomanufacturing from mega-scale towards smaller efficient processes. To select the best of many candidates, tools for clone screening, selection and scaling up have become more important than ever. The ready availability of high-expressing cell lines makes it possible to use incubation shakers instead of bioreactors for many bioproduction needs. Incubation shakers are already standard issue for cell line screening and development. Therefore, they provide a simple and easily validated path from clone screening all the way to cGMP manufacturing.
同步哺乳动物细胞培养：第 IâA 部分生理条件下同步培养的物理策略
影响因子：
2.9
作者：
Platas Barradas;Becker;Bahnemann;Pörtner
通讯作者：
Pörtner
同步哺乳动物细胞培养：第二部分：群体整体建模和分析，用于开发可重复的过程
影响因子：
2.9
作者：
Platas Barradas;Pörtner
通讯作者：
Pörtner