Interfacial chemistry of the dentin/adhesive bond
Interfacial chemistry of the dentin/adhesive bond
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DOI：
10.1177/00220345000790070501
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发表时间：
2000-07-01
影响因子：
7.6
通讯作者：
Swafford, JR
中科院分区：
文献类型：
--
作者：
Spencer, P;Wang, Y;Swafford, JR
迄今为止，牙本质/粘合剂（d/a）粘结主要是通过结合粘结强度测量的形态学分析来研究的。虽然这些分析增强了我们的理解，但关于化学的许多问题尚未得到解答。本研究的目的是确定，在分子水平上，定量差异的组成的d/a界面下形成的“湿”键合条件。从10颗拔除的未萌出的人类第三磨牙中取出咬合三分之一的牙冠。根据制造商的说明书，用Single Bond（3 M）或One-Step粘合剂（Bisco）处理制备的牙本质表面。切割d/a界面的三微米厚切片并用Goldner三色染色用于光学显微镜检查。伴随板坯进行了分析与显微拉曼光谱，样品被放置在一个100倍显微镜物镜的焦点，并获得光谱在1 μ m的间隔跨越d/a接口。收集I型胶原蛋白和粘合剂的模型化合物的参考光谱;确定粘合剂和胶原蛋白的光谱特征的积分强度的相对比率，并将其绘制为粘合剂重量%的函数。相同的比例确定的接口样品，通过比较这些比例与模型化合物产生的校准曲线，我们确定了粘合剂的百分比作为整个d/a接口的空间位置的函数。在超过一半的混合层中，Single Bond粘合剂的相对百分比< 50%;在大部分混合层中，One Step粘合剂大于或等于50%。这项研究的结果提供了第一个直接的化学证据相分离的牙本质粘合剂和它的不利影响的牙本质/粘合剂粘结。
To date, the dentin/adhesive (d/a) bond has primarily been studied by morphologic analysis in conjunction with bond strength measurement. Although these analyses have enhanced our understanding, numerous questions about the chemistry have not been answered. The purpose of this study was to determine, at the molecular level, quantitative differences in the composition of the d/a interface formed under "wet" bonding conditions. The occlusal one-third of the crown was removed from 10 extracted, unerupted human third molars. The prepared dentin surfaces were treated, per manufacturers' instructions, with either Single Bond (3M) or One-Step adhesive (Bisco). Three-micron-thick sections of the d/a interface were cut and stained with Goldner trichrome for light microscopy. Companion slabs were analyzed with micro-Raman spectroscopy; the sample was placed at the focus of a 100x microscope objective, and spectra were acquired at 1-mu m intervals across the d/a interface. Reference spectra were collected on model compounds of type I collagen and adhesive; the relative ratios of the integrated intensities of spectral features from adhesive and collagen were determined and plotted as a function of wt% adhesive. The same ratios were determined for the interface samples; by comparing these ratios with the calibration curve generated from the model compounds, we determined the percent of adhesive as a function of spatial position across the d/a interface. The relative percent of Single Bond adhesive was < 50% throughout more than half of the hybrid layer; One Step adhesive was greater than or equal to 50% throughout most of the hybrid. The results from this study provide the first direct chemical evidence of phase separation in a dentin adhesive and its detrimental effect on the dentin/adhesive bond.