Effective gene delivery with liposomal bubbles and ultrasound as novel non-viral system
Effective gene delivery with liposomal bubbles and ultrasound as novel non-viral system
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DOI：
10.1080/10611860701499789
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发表时间：
2007-01-01
影响因子：
4.5
通讯作者：
Maruyama, Kazuo
中科院分区：
文献类型：
--
作者：
Suzuki, Ryo;Takizawa, Tomoko;Maruyama, Kazuo
我们研制了含有超声成像气体全氟丙烷的新型脂质体气泡（Bubble liposomes）。气泡脂质体由聚乙二醇化脂质体制成，比常规微泡更小。气泡脂质体在心脏声像学中也有显像剂的作用。此外，气泡脂质体可以结合超声将质粒DNA传递到体外各种类型的细胞中，而不会产生细胞毒性。在体内基因传递中，气泡脂质体可以通过超声经皮暴露将质粒DNA传递到小鼠股动脉。转染效率高于脂质转染法。有趣的是，基因表达仅在超声暴露部位观察到。因此，我们认为气泡脂质体可以作为建立组织特异性基因传递系统的良好工具和超声显像剂。
We developed the novel liposomal bubbles (Bubble liposomes) containing ultrasound imaging gas, perfluoropropane. Bubble liposomes were made of pegylated liposomes and were smaller than conventional microbubbles. Bubble liposomes also had a function as imaging agents in cardiosonography. In addition, Bubble liposomes could deliver plasmid DNA into various types of cells in vitro without cytotoxicity by the combination of ultrasound. In vivo gene delivery, Bubble liposomes could deliver plasmid DNA into mouse femoral artery by the transdermally exposure of ultrasound. This transfection efficiency was more effectively than lipofection method. Interestingly, the gene expression was only observed at the site of ultrasound exposure. Therefore, we concluded that Bubble liposomes could be good tools to establish tissue-specific gene delivery system as well as ultrasound imaging agents.