Effect of Bacterial Flora and Mouse Genotype (Euthymic or Athymic) on Scrapie Pathogenesis
Effect of Bacterial Flora and Mouse Genotype (Euthymic or Athymic) on Scrapie Pathogenesis
复制标题
细菌菌群和小鼠基因型（正常或无胸腺）对痒病发病机制的影响
DOI：
10.1002/jlb.40.5.525
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发表时间：
1986
影响因子：
5.5
通讯作者：
R. Marsh
中科院分区：
文献类型：
--
作者：
W. Wade;C. Dees;T. German;R. Marsh
在无菌或特定植物群条件下饲养的正常胸腺和无胸腺雌性BALB/c小鼠，在脑内或腹腔内接种后用于研究瘙痒症的发病机制。比较各组间至出现临床体征（I期）、至终末期（II期）的时间（天）以及I期和II期之间的时间间隔。此外，在感染后28天和90天测定脾脏中的痒病病原体滴度，以及在第II阶段测定脾脏和脑中的病原体滴度。三因素方差分析表明，细菌植物群、胸腺的存在或不存在以及接种途径相互作用，在疾病发病机制中产生显著差异。实验设计中的三个因素也影响羊瘙痒病感染性的脾滴度。各组小鼠之间瘙痒病发病机制的变化可能是由它们的网状内皮系统的差异介导的。这些差异可能会改变药物在脾脏中的吸附和/或从脾脏到大脑的转运途径。
Euthymic and athymic female BALB/c mice, reared under either germfree or defined flora conditions, were used to investigate the pathogenesis of scrapie after intracerebral or intraperitoneal inoculation. Time in days to onset of clinical signs (Stage I), to endstage (Stage II), and the time interval between Stage I and Stage II were compared among groups. In addition, scrapie agent titers in spleen were determined at 28 and 90 days after infection, as were agent titers in spleen and brain at Stage II. Three‐way analysis of variance indicated that the bacterial flora, the presence or absence of a thymus, and the route of agent inoculation interact to produce significant differences in the pathogenesis of disease. The three factors in the experimental design also influenced the spleen titers of scrapie infectivity. The variation in scrapie pathogenesis among the groups of mice is likely to be mediated by differences in their reticuloendothelial systems. These differences may alter the agent's adsorption in spleen and/or route of transport from spleen to brain.