Gold nanoparticle mediated method for spatially resolved deposition of DNA on nano-gapped interdigitated electrodes, and its application to the detection of the human Papillomavirus
Gold nanoparticle mediated method for spatially resolved deposition of DNA on nano-gapped interdigitated electrodes, and its application to the detection of the human Papillomavirus
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DOI：
10.1007/s00604-016-1954-9
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发表时间：
2016-12-01
影响因子：
5.7
通讯作者：
Nadzirah, Sharipah
中科院分区：
文献类型：
--
作者：
Azizah, Nor;Hashim, Uda;Nadzirah, Sharipah
作者介绍了一种方法，空间排列的DNA固定在10纳米金纳米粒子（GNP）沉积在硅基板携带纳米间隙叉指电极。GNP通过硅烷化学共价结合到表面，并且通过FTIR光谱和原子力显微镜监测制造的单个步骤。这种GNP沉积技术显示出将纳米间隙的尺寸减小到130 nm。FTIR还用于监测DNA在叉指电极表面上的固定。该方法允许DNA以均匀和均质的方式固定。通过将探针DNA固定在表面上并通过荧光检测对人乳头状瘤病毒特异的靶DNA来证明该方法的实用性，检测限低至1 pM。在我们看来，这种用于GNP介导的DNA固定化的方法能够高性能地感测广泛的目标（分析物）DNA。
The authors introduce a method for spatially arranged DNA immobilization on 10-nm gold nanoparticles (GNP) deposited on a silicon substrate carrying nanogapped interdigitated electrodes. The GNPs are covalently bound to the surface via silane chemistry, and the single steps of fabrication are monitored by FTIR spectroscopy and atomic force microscopy. This GNP deposition technique is shown to reduce the size of the nanogaps to 130 nm. FTIR also was used to monitor the immobilization of DNA on the surface of the interdigitated electrodes. This method allows DNA to be immobilized in a uniform and homogenous way. The utility of the method is demonstrated by immobilizing probe DNA on the surface and detecting target DNA specific for the human papilloma virus via fluorescence with a detection limit as low as 1 pM. In our perception, this method for GNP-mediated DNA immobilization enables high-performance sensing of a wide range of target (analyte) DNA.