Structural and functional analysis of 3′ untranslated region of mouse Period1 mRNA
Structural and functional analysis of 3′ untranslated region of mouse Period1 mRNA
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DOI：
10.1016/s0006-291x(02)02938-8
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发表时间：
2003-01-31
影响因子：
3.1
通讯作者：
Tei, H
中科院分区：
文献类型：
--
作者：
Kojima, S;Hirose, M;Tei, H
为了研究Period 1（Per 1）的转录后调控，对小鼠Per 1（mPer 1）mRNA的3 '非翻译区（3' UTR）进行了分析。除了人和小鼠Per 1 3 'UTR之间高度相似之外，在两个物种中还发现了富含AU的元件和分化控制元件。使用luc：：mPer 1 3 'UTR融合基因的瞬时转染测定揭示了mPer 1 3' UTR以转录后方式抑制其自身的表达。对这种翻译下调至关重要的区域被限制在核苷酸位置322-517。这些结果提示mPer 1的3 'UTR可能参与了mPer 1在视交叉上核中转录和翻译产物之间的时滞的产生。(C)2002 Elsevier Science（美国）。All rights reserved.
In order to investigate the post-transcriptional regulation of Period1 (Per1), the 3'-untranslated region (3'UTR) of mouse Per1 (mPer1) mRNA was characterized. In addition to high similarity between human and mouse Per1 3'UTRs, AU-rich element and differentiation control element were found in both species. Transient transfection assays using luc::mPer1 3'UTR fusion genes revealed that the mPer1 3'UTR repressed its own expression in a post-transcriptional manner. The region critical for this translational down-regulation was confined to nucleotide positions 322-517. These results suggest that the mPer1 3'UTR could be involved in the generation of time lag between the transcriptional and translational products of mPer1 in the suprachiasmatic nucleus. (C) 2002 Elsevier Science (USA). All rights reserved.