Engineering an Injectable Electroactive Nanohybrid Hydrogel for Boosting Peripheral Nerve Growth and Myelination in Combination with Electrical Stimulation
Engineering an Injectable Electroactive Nanohybrid Hydrogel for Boosting Peripheral Nerve Growth and Myelination in Combination with Electrical Stimulation
复制标题
设计可注射的电活性纳米混合水凝胶，结合电刺激促进周围神经生长和髓鞘形成
DOI：
10.1021/acsami.0c16885
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发表时间：
2020-11-25
影响因子：
9.5
通讯作者：
Ramakrishna, Seeram
中科院分区：
文献类型：
--
作者：
He, Liumin;Xiao, Qiao;Ramakrishna, Seeram
电刺激（ES）可用于操纵周围神经损伤后的恢复。尽管基于生物材料的策略已经被实施，以获得ES和工程允许的神经再生微环境的动力，但开发用于特定刺激响应神经细胞特性调节的生物材料仍然是一个挑战。在此，我们将原始碳纳米管均匀地掺入功能性自组装肽中，以制备具有良好注射性和导电性的混合水凝胶。二维（表面）和三维（混合水凝胶内）培养实验表明，ES促进轴突生长和雪旺细胞（SC）远离背根神经节球，进一步揭示了ES增强SC与轴突之间的相互作用导致髓鞘形成改善。因此，我们的研究不仅促进了定制材料的发展，而且为促进神经生长的综合方法提供了有用的见解，并提出了修复周围神经损伤的实用策略。
Electrical stimulation (ES) can be used to manipulate recovery after peripheral nerve injuries. Although biomaterial-based strategies have already been implemented to gain momentum for ES and engineer permissive microenvironments for neural regeneration, the development of biomaterials for specific stimuli-responsive modulation of neural cell properties remains a challenge. Herein, we homogeneously incorporate pristine carbon nanotubes into a functional self-assembling peptide to prepare a hybrid hydrogel with good injectability and conductivity. Two-dimensional (on the surface) and three-dimensional (within the hybrid hydrogel) culturing experiments demonstrate that ES promotes axon outgrowth and Schwann cell (SC) migration away from dorsal root ganglia spheres, further revealing that ES-enhanced interactions between SCs and axons result in improved myelination. Thus, our study not only advances the development of tailor-made materials but also provides useful insights into comprehensive approaches for promoting nerve growth and presents a practical strategy of repairing peripheral nerve injuries.