Modulation of colonic barrier function by the composition of the commensal flora in the rat
Modulation of colonic barrier function by the composition of the commensal flora in the rat
复制标题
DOI：
10.1136/gut.48.4.503
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发表时间：
2001-04-01
期刊：
影响因子：
24.5
通讯作者：
Malagelada, JR
中科院分区：
文献类型：
--
作者：
García-Lafuente, A;Antolín, M;Malagelada, JR
背景和目的：肠道通透性改变是特发性肠道炎症的一个重要致病因素。我们调查了在大鼠的肠道植物群的组成的变化，如果可以改变结肠permeability. Methods-A结肠段手术排除粪便运输，并通过两个colostomies作为一个循环到腹壁。在用抗生素根除天然植物群后，将该环用于特定细菌菌株的定殖。在用来自大鼠结肠来源的单一细菌菌株接种的大鼠和通过抗生素保持结肠环无细菌的对照大鼠中，测量葡聚糖（分子量70 000）和甘露醇（分子量182）的管腔血液清除率。实际的殖民地被证实培养段effluents. Results-大肠杆菌，肺炎克雷伯菌，草绿色链球菌显着增加管腔甘露醇的血液清除率的殖民地。用短乳杆菌定殖具有相反的效果，并降低了对甘露醇的渗透性。脆弱拟杆菌未引起显著变化。葡聚糖的渗透性没有改变任何测试strains. Conclusions某些肠道细菌可以修改结肠壁的通透性管腔物质。
Background and aims-Altered intestinal permeability is a key pathogenetic factor of idiopathic bowel inflammation. We investigated in the rat if changes in the composition of the bowel flora can alter colonic permeability.Methods-A colonic segment was surgically excluded from faecal transit and brought out as a loop to the abdominal wall through two colostomies. The loop was used for colonisation with specific bacterial strains after eradication of the native flora with antibiotics. Lumen to blood clearance of dextran (molecular weight 70 000) and mannitol (molecular weight 182) was measured in rats recolonised with a single bacterial strain from rat colonic origin, and in control rats whose colonic loop was kept free of bacteria by antibiotics. Actual colonisation was confirmed by culture of segment effluents.Results-Colonisation with Escherichia coli, Klebsiella pneumoniae, and Streptococcus viridans significantly increased lumen to blood clearance of mannitol. Colonisation with Lactobacillus brevis had the opposite effect and reduced permeability to mannitol. Bacteroides fragilis did not induce significant changes. Permeability to dextran was not altered by any of the strains tested.Conclusions-Certain commensal bacteria can modify colonic wall permeability to luminal substances.