Propagule Powder of Japanese Yam (Dioscorea Japonica) Reduces High‐Fat Diet‐Induced Metabolic Stress in Mice through the Regulation of Hepatic Gene Expression
Propagule Powder of Japanese Yam (Dioscorea Japonica) Reduces High‐Fat Diet‐Induced Metabolic Stress in Mice through the Regulation of Hepatic Gene Expression
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日本山药（Dioscorea Japonica）繁殖体粉通过调节肝脏基因表达减少高脂饮食引起的小鼠代谢应激
DOI：
10.1002/mnfr.202000284
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发表时间：
2020
影响因子：
5.2
通讯作者：
Abe Keiko
中科院分区：
文献类型：
--
作者：
Shinozaki Fumika;Kamei Asuka;Watanabe Yuki;Yasuoka Akihito;Shimada Kousuke;Kondo Kaori;Arai Soichi;Kondo Takashi;Abe Keiko
范围日本山药繁殖体被认为具有很高的潜力作为一种功能性食品。然而，几乎没有研究检查其生理功能。本研究旨在阐明日本山药繁殖体的生理功能，加热，冷冻干燥，和powdered.Methods和resultsA高脂肪饮食与日本山药繁殖体给小鼠4周。高脂肪负荷会导致呼吸商下降，而含有繁殖体的高脂肪饮食会进一步降低呼吸商。这一结果表明，繁殖体增加脂肪氧化，表明脂肪利用。使用DNA微阵列分析肝转录组。一些受高脂肪负荷影响的基因通过同时摄入繁殖体而逆转。这些基因主要参与免疫系统和脂肪代谢。高脂肪负荷诱导肝脏炎症，其通过同时摄入繁殖体来抑制。对于脂质代谢，繁殖体通过高脂肪负荷抑制胆固醇生物合成和催化剂的增加。在碳水化合物代谢方面，繁殖体减少糖酵解和糖原合成，增加糖原合成。此外，氨基酸被转化为丙酮酸，然后被用于肝脏的再生。结论在高脂负荷的小鼠中，糖和脂肪代谢紊乱可通过抑制糖和脂肪代谢紊乱来延缓肝脏疾病的发生。
ScopeJapanese yam propagules are supposed to have high potential as a functional food. However, there are almost no studies examining their physiological function. This study aims to elucidate the physiological function of Japanese yam propagules that are heated, freeze‐dried, and powdered.Methods and resultsA high‐fat diet with Japanese yam propagules is administered to mice for 4 weeks. High‐fat loading induces a decline in respiratory quotient, and a high‐fat diet with propagules reduces it more. This result suggests that propagules increase fat oxidation, indicating fat utilization. The hepatic transcriptome is analyzed using a DNA microarray. Some of the genes affected by high‐fat loading are reversed by simultaneous ingestion of propagules. Such genes are mainly involved in the immune system and fat metabolism. High‐fat loading induces hepatic inflammation, which is repressed by simultaneous ingestion of propagules. For lipid metabolism, propagules repress an increase in cholesterol biosynthesis and catabolism by high‐fat loading. Regarding carbohydrate metabolism, propagules decrease glycolysis and glycogen synthesis and increase gluconeogenesis. Moreover, amino acids are converted into pyruvate and then used for gluconeogenesis.ConclusionPropagules act to delay the occurrence of hepatic disease by suppressing carbohydrate and fat metabolism disorders in high‐fat loaded mice.