Detection and characterization of Shiga toxin-producing Escherichia coli in captive non-domestic mammals
Detection and characterization of Shiga toxin-producing Escherichia coli in captive non-domestic mammals
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DOI：
10.1016/j.vetmic.2006.07.006
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发表时间：
2006-11-26
影响因子：
3.3
通讯作者：
Rivas, Marta
中科院分区：
文献类型：
--
作者：
Leotta, Gerardo A.;Deza, Natalia;Rivas, Marta
产志贺毒素大肠杆菌（STEC）是一种重要的新兴病原体，反刍动物被认为是其主要的天然宿主。本研究的目的是确定产志贺毒素大肠杆菌在阿根廷拉普拉塔市动物园和植物园的非家养哺乳动物中的频率，并对产志贺毒素大肠杆菌分离株进行表型基因型表征。聚合酶链反应（PCR）检测65份粪便中志贺毒素（stx）基因序列的阳性率为50.8%。从38.5%的动物中分离出25株产志贺毒素大肠杆菌。10种鲸鱼目和1种啮齿目被确认为产志贺毒素大肠杆菌的新携带者。产志贺毒素大肠杆菌分7种不同的血清型，包括新的血清型012:H25和013:H6。血清型0146:H28，以前与人类感染相关，占产志贺毒素大肠杆菌分离株的24%。最常见的志贺毒素是I型、c型和2c型。iha基因阳性19株，ehxA基因阳性8株。所有菌株的lpfA（O113）基因均呈阳性，rfb0157、eae、saa、lpfA（O157/OI-141）、lpfA（O157/OI-154）、efa1和toxB基因均呈阴性。xbai -脉冲场凝胶电泳（XbaI-PFGE）结果证实了产志毒素大肠杆菌菌株在不同动物种类和亚目之间的传播。此外，我们还观察到携带stec的动物与鲸鱼目、生活在坑中、属于非本土物种等因素之间的潜在关联。这是阿根廷首次利用动物哺乳动物和产志贺毒素大肠杆菌开展的工作。(c) 2006 Elsevier B.V.版权所有
Shiga toxin producing-Escherichia coli (STEC) is an important emerging pathogen, and ruminants are recognized as their main natural reservoir. The aim of this work was to establish the frequency of STEC in non-domestic mammals of the Zoo and Botanical Garden of La Plata City, Argentina, and to pheno-genotypically characterize STEC isolates. By polymerase chain reaction (PCR), Shiga toxin (stx) gene sequences were detected in 50.8% of 65 fecal samples. Twenty-five STEC strains were isolated from 38.5% of the Zoo's animals. Ten species of order Cetartiodactyla and one species of order Rodentia were recognized as new STEC carriers. STEC strains belonged to 7 different serotypes including new serotypes 012:H25 and 013:H6. Serotype 0146:H28, previously associated with human infections, represented 24% of STEC isolates. The most frequent Shiga toxin identified were type I c and type 2c. Nineteen strains were positive for iha gene, 8 strains were positive for ehxA gene. Moreover, all strains were positive for lpfA(O113) and negative for rfb0157, eae, saa, lPfA(O157/OI-141), lPfA(O157/OI-154), efa1, and toxB genes. Results obtained by XbaI-pulsed-field gel electrophoresis (XbaI-PFGE) confirmed the transmission of STEC strains among different animal species and suborders. In addition, we observed a potential association between STEC-harboring animal and factors such as belonging to order Cetartiodactyla, living in a pit, and belonging to a non-autochthonous species. This is the first work developed with zoological mammals and STEC in Argentina. (c) 2006 Elsevier B.V. All rights reserved.