Conserved regulation of Math5 and Math1 revealed by Math5-GFP transgenes
Conserved regulation of Math5 and Math1 revealed by Math5-GFP transgenes
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DOI：
10.1016/j.mcn.2007.08.006
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发表时间：
2007-12-01
影响因子：
3.5
通讯作者：
Brown, Nadean L.
中科院分区：
文献类型：
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作者：
Hufnagel, Robert B.;Riesenberg, Amy N.;Brown, Nadean L.
视网膜神经节细胞的发生需要前神经bHLH转录因子Math 5（Atoh 7），但鲜为人知的是，控制其表达的调控元件。在这里，我们使用转基因小鼠研究Math 5基因调控。这些小鼠在产前视网膜中表达GFP，活标记RGC轴突迁移和脑神经支配。出乎意料的是，这些Math 5-GFP转基因也在整个神经系统的Math 1表达域中发现，这很有趣，因为Math 5和Math 1通常表现出不重叠的表达。此外，Math 5-GFP和Moth]在下菱形唇和背侧脊髓中受到Pax 6和Math 1本身的类似调节。我们还表明，Pax 6结合特定的Math 5和Math 1调控序列在体外。总之，这些数据表明，这些无调半直向同源物可能共享通常在Math 5基因中沉默的保守调控元件。(c)2007年爱思唯尔公司All rights reserved.
Retinal ganglion cell genesis requires the proneural bHLH transcription factor Math5 (Atoh7), but little is known about the regulatory elements that control its expression. Here, we investigate Math5 gene regulation using transgenic mice. These mice express GFP in the prenatal retina, live-labeling RGC axon migration and innervation of the brain. Unexpectedly, these Math5-GFP transgenes are also found in Math1 expression domains throughout the nervous system, intriguing since Math5 and Math1 normally exhibit nonoverlapping expression. Furthermore, Math5-GFP and Moth] are regulated similarly, by both Pax6 and Math1 itself, in the lower rhombic lip and dorsal spinal cord. We also show that Pax6 binds to particular Math5 and Math1 regulatory sequences in vitro. Together these data suggest that these atonal semi-orthologues may share conserved regulatory elements that are normally silent in the Math5 gene. (c) 2007 Elsevier Inc. All rights reserved.