Quantitation of tamoxifen, 4-hydroxytamoxifen, and N- desmethyltamoxifen in human plasma by high performance liquid chromatography
Quantitation of tamoxifen, 4-hydroxytamoxifen, and N- desmethyltamoxifen in human plasma by high performance liquid chromatography
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高效液相色谱法定量人血浆中他莫昔芬、4-羟基他莫昔芬和 N-去甲基他莫昔芬
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
M. Degregorio
中科院分区：
文献类型：
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作者：
B. Wilbur;C. Benz;M. Degregorio
摘要建立了一种从人血浆中提取的三苯氧胺、4-羟基三苯氧胺和N-去甲基三苯氧胺的反相高效液相色谱定量分析方法。血浆样品加标内标物萘福昔定，用2%丁醇的己烷溶液提取，并通过暴露于高强度短波紫外线（254 nm）激活荧光。然后将提取血浆的等分试样进样至C18反相色谱柱上，并用水和三乙胺的甲醇溶液移动的流动相等度洗脱。在266 nm处测量活化的他莫昔芬及其代谢物的荧光。使用峰高比定量提取的人血浆标本中的他莫昔芬及其代谢产物。
Abstract A reverse phase high performance liquid chromatography assay used for quantitative analysis of tamoxifen, 4-hydroxytamoxifen, and N-desmethyltamoxifen extracted from human plasma was developed. Plasma samples were spiked with an internal standard, nafoxidine, extracted with 2% butanol in hexane, and activated to fluorescence by exposure to high intensity short-wave ultraviolet (254 nm) light. Aliquots of extracted plasma were then injected onto a C18 reverse phase column and eluted isocratically with a mobile phase of water and triethylamine in methanol. Fluorescence of activated tamoxifen and its metabolites was measured at 266 nm. Peak height ratios were used to quantitate tamoxifen and its metabolites from extracted human plasma specimens.