Differential tissue expression of the Lewis blood group antigens: enzymatic, immunohistologic, and immunochemical evidence for Lewis a and b antigen expression in Le(a-b-) individuals.
Differential tissue expression of the Lewis blood group antigens: enzymatic, immunohistologic, and immunochemical evidence for Lewis a and b antigen expression in Le(a-b-) individuals.
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DOI：
10.1182/blood.v77.6.1389.bloodjournal7761389
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发表时间：
1991-03
期刊：
影响因子：
20.3
通讯作者：
T. Orntoft;Eric H. Holmes;Philip J. Johnson;Sen-itiroh Hakomori;Henrik Clausen
中科院分区：
文献类型：
--
作者：
T. Orntoft;Eric H. Holmes;Philip J. Johnson;Sen-itiroh Hakomori;Henrik Clausen
Lewis血型系统包括两个主要的碳水化合物抗原，Le(A)和Le(B)。Lewis分型传统上是基于红细胞和唾液的血清学测定。最近的几项研究表明，在妊娠或疾病期间，红细胞Lewis表型可能会发生变化，在正常组织和肿瘤组织中都发现了不适当的Lewis抗原。为了评估这些观察结果是否与目前提出的Lewis血型系统的遗传和生物合成基础相冲突，我们对正常组织和肿瘤组织以及Le(a-b)型个体的红细胞、血浆和唾液中Lewis抗原的表达进行了酶、免疫组织学和免疫化学联合研究。在6例Le(a-b-)型癌症患者中，3例由于唾液中存在α1-4岩藻糖基转移酶活性(α1-4ft)和Lewis抗原而被鉴定为假阳性，3例被鉴定为真阳性(唾液中缺乏α1-4ft和Lewis抗原)。这些真正的Le(a-b-)人被证明在组织中表达显著的α1-4ft，免疫组织学和免疫化学在组织中检测到Lewis抗原。我们的结论是，通过红细胞和唾液的血清学测定获得的Lewis表型并不适用于所有组织。我们讨论了这一发现的生物合成和遗传后果。
The Lewis blood group system comprises two main carbohydrate antigens, Le(a) and Le(b). Lewis typing has traditionally been based on serologic determinations using erythrocytes and saliva. Several recent studies have demonstrated that erythrocyte Lewis phenotype may change during pregnancy or disease, and inappropriate Lewis antigens have been found in both normal and neoplastic tissue. To evaluate whether these observations are in conflict with the presently proposed genetic and biosynthetic basis of the Lewis blood group system, we performed a combined enzymatic, immunohistologic, and immunochemical study of Lewis antigen expression in normal and neoplastic tissues, as well as erythrocytes, plasma, and saliva of Le(a-b-)-typed individuals. Of six cancer-bearing patients typed Le(a-b-), three were identified as nongenuine owing to the presence of alpha 1----4fucosyltransferase activity (alpha 1----4FT) and Lewis antigens in saliva and three were identified as genuine (lacking alpha 1----4FT and Lewis antigens in saliva). These genuine Le(a-b-) individuals were shown to express significant alpha 1----4FT in tissues, and Lewis antigens were detected in tissues by immunohistology as well as immunochemistry. We conclude that the Lewis phenotype obtained by serologic determination of erythrocytes and saliva does not apply to all tissues. We discuss biosynthetic and genetic consequences of this finding.