A Preliminary Study Examining the Binding Capacity of Akkermansia muciniphila and Desulfovibrio spp., to Colonic Mucin in Health and Ulcerative Colitis.
A Preliminary Study Examining the Binding Capacity of Akkermansia muciniphila and Desulfovibrio spp., to Colonic Mucin in Health and Ulcerative Colitis.
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DOI：
10.1371/journal.pone.0135280
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
O'Connell PR
中科院分区：
文献类型：
--
作者：
Earley H;Lennon G;Balfe A;Kilcoyne M;Clyne M;Joshi L;Carrington S;Martin ST;Coffey JC;Winter DC;O'Connell PR
粘性阿克曼氏菌和脱硫弧菌。是寄生在结肠粘液凝胶层的共生微生物。这两个物种都有能力利用结肠粘蛋白作为底物。嗜粘杆菌能降解结肠粘蛋白，而硫弧菌能降解结肠粘蛋白。代谢硫酸盐化粘蛋白的硫酸盐部分。据报道，溃疡性结肠炎(UC)中这些微生物的数量发生了变化。然而，它们与人结肠粘蛋白的结合能力，以及这种结合能力是否受到UC相关粘蛋白变化的影响，仍有待确定。分别从7例结肠癌切除患者和5例UC切除患者的切除结肠中分离出粘蛋白。分离的粘蛋白纯化后印迹于粘蛋白微阵列上。粘液弧菌和脱硫弧菌三株临床分离株与参考菌株的结合对纯化粘蛋白进行了研究。粘液拟青霉和脱硫菌都是。与粘蛋白结合。参比菌株和所有临床分离株对UC粘蛋白的结合能力均增强(p<；.005)。脱硫弧菌参比菌株对UC粘蛋白的亲和力增强。脱硫弧菌临床分离株的粘蛋白结合谱。是每个分离物所特有的。两个分离株在结合力上没有差异。一株与UC粘蛋白亲和力增强的UC分离物(p<；.005)。这些初步数据表明，粘液弧菌和硫化弧菌分离株的粘蛋白结合能力存在差异。这项研究强调了粘蛋白微阵列平台作为一种研究细菌与结肠粘蛋白在健康和疾病中相互作用的能力的手段。
Akkermansia muciniphila and Desulfovibrio spp. are commensal microbes colonising the mucus gel layer of the colon. Both species have the capacity to utilise colonic mucin as a substrate. A. muciniphila degrades colonic mucin, while Desulfovibrio spp. metabolise the sulfate moiety of sulfated mucins. Altered abundances of these microorganisms have been reported in ulcerative colitis (UC). However their capacity to bind to human colonic mucin, and whether this binding capacity is affected by changes in mucin associated with UC, remain to be defined. Mucin was isolated from resected colon from control patients undergoing resection for colonic cancer (n = 7) and patients undergoing resection for UC (n = 5). Isolated mucin was purified and printed onto mucin microarrays. Binding of reference strains and three clinical isolates of A. muciniphila and Desulfovibrio spp. to purified mucin was investigated. Both A. muciniphila and Desulfovibro spp. bound to mucin. The reference strain and all clinical isolates of A. muciniphila showed increased binding capacity for UC mucin (p < .005). The Desulfovibrio reference strain showed increased affinity for UC mucin. The mucin binding profiles of clinical isolates of Desulfovibrio spp. were specific to each isolate. Two isolates showed no difference in binding. One UC isolate bound with increased affinity to UC mucin (p < .005). These preliminary data suggest that differences exist in the mucin binding capacity of isolates of A. muciniphila and Desulfovibrio spp. This study highlights the mucin microarray platform as a means of studying the ability of bacteria to interact with colonic mucin in health and disease.