Hysteresis in the Cell Response to Time-Dependent Substrate Stiffness
Hysteresis in the Cell Response to Time-Dependent Substrate Stiffness
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DOI：
10.1016/j.bpj.2010.04.008
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发表时间：
2010-07-07
影响因子：
3.4
通讯作者：
Schwarz, Ulrich S.
中科院分区：
文献类型：
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作者：
Besser, Achim;Schwarz, Ulrich S.
机械线索，如基板的刚度是粘附细胞决策的主要决定因素。在这里，我们使用机械化学模型来预测细胞对不同基质硬度的反应。模型方程结合联合收割机的力学收缩肌动蛋白纤维束与一个模型的Rho信号通路触发的部队在细胞-基质接触。作为基底刚度的函数的细胞收缩性的分叉分析揭示了一种非线性响应，从而定义了刚度的下限阈值，低于该下限阈值，细胞不能建立收缩力，以及刚度的上限阈值，高于该上限阈值，细胞总是处于强烈收缩状态。使用完整的动力学模型，我们预测，率相关的滞后将发生在细胞的牵引力时，细胞暴露于基板的时间依赖性刚度。
Mechanical cues like the rigidity of the substrate are main determinants for the decision-making of adherent cells. Here we use a mechano-chemical model to predict the cellular response to varying substrate stiffnesses. The model equations combine the mechanics of contractile actin filament bundles with a model for the Rho-signaling pathway triggered by forces at cell-matrix contacts. A bifurcation analysis of cellular contractility as a function of substrate stiffness reveals a bistable response, thus defining a lower threshold of stiffness, below which cells are not able to build up contractile forces, and an upper threshold of stiffness, above which cells are always in a strongly contracted state. Using the full dynamical model, we predict that rate-dependent hysteresis will occur in the cellular traction forces when cells are exposed to substrates of time-dependent stiffness.