Neocortical Neurogenesis in Development and Evolution
Neocortical Neurogenesis in Development and Evolution
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发育和进化中的新皮质神经发生
DOI：
10.1002/9781119860914.ch16
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Henning T
中科院分区：
文献类型：
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作者：
Henning T
在所有哺乳动物中，早期产生的和大量短暂的皮质亚板神经元执行重要的发育功能。然而，这种细胞群中只有一个亚群在小鼠中作为层6 b（L 6 b）存活到成年，在人类中作为间质白色细胞存活到成年。L 6 b由不同的细胞群组成，小鼠L 6 b细胞的各种特征已被单独研究。在这里，我们的目标是结合联合收割机转录组聚类的细胞基于单细胞基因表达与更传统的数据，以达到一个统一的细胞分类，通过全面Meta分析。然而，将各种解剖学和生理学参数与基于单细胞转录组数据集的分类相匹配是复杂的。在这里，我们探讨这些困难的根源。我们的分析揭示了多巴胺能L 6 b类Slc 17 a8（317），其由于其独特的基因表达、全局定位和食欲素/下丘脑泌素受体2（Hcrtr 2）的表达而突出。我们概述了未来的研究需要采用多种方法来生成全等聚类。我们建议必须重新评估每个维度的权重，转录组数据需要半独立聚类。
Early generated and largely transient cortical subplate neurons perform important developmental functions in all mammals. However, only a subset of this cell population survives into adulthood as layer 6b (L6b) in mouse and interstitial white matter cells in human. L6b is comprised of a diverse population of cells and various characteristics of mouse L6b cells have been investigated individually. Here, our aim is to combine transcriptomic clustering of cells based on single cell gene expression with more conventional data to arrive at a unified cell taxonomy via a comprehensive meta‐analysis. However, matching up various anatomical and physiological parameters with the classification based on single cell transcriptomic datasets is complex. Here we explore the origins of these difficulties. Our analysis revealed glutamatergic L6b class Slc17a8 (317), which stands out due to its unique gene expression, global localization, and expression of orexin/hypocretin receptor 2 (Hcrtr2). We outline the need of future studies to employ multiple approaches to generate congruent clustering. We propose that the weight of each dimension must be revaluated, with transcriptomic data requiring semi‐independent clustering.