Evasion of encapsulation by the polyembryonic parasitoid Copidosoma floridanum is mediated by a polar body-derived extraembryonic membrane
Evasion of encapsulation by the polyembryonic parasitoid Copidosoma floridanum is mediated by a polar body-derived extraembryonic membrane
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DOI：
10.1016/s0022-2011(03)00041-7
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发表时间：
2003-05-01
影响因子：
3.4
通讯作者：
Strand, MR
中科院分区：
文献类型：
--
作者：
Corley, LS;Strand, MR
从实验室饲养的pseudoplusia包括[Chrysodeixis包括]和trichoplusia nih中分离出的C. floridanum1龄幼虫在有或没有疾病的磷酸盐缓冲盐水（PBS; 7.2 pH）中孵育30分钟（1 U/100µl）。PBS中的幼虫被膜包裹住。PBS冲洗后，将幼虫接种于容许型（P. inclenst）和t。i)和非寄主（Spodoptera frugiperda， Heliothis virescens和manduca sexta）种。接种24 h后解剖所有寄主，用相差显微镜测定被包膜的幼虫数量。总的来说，合胞膜的存在与否对所有寄主物种的包封率都有很大的影响。没有胚外膜的幼虫被包裹，与寄主种类无关。被膜包裹的幼虫几乎没有被p包裹。includensorT。但非寄主种被包衣的幼虫比例越来越高。被合胞膜包裹的幼虫对p的包封反应最弱。includensandT。ni是h的中间反应。绿色植物，在m。sextaandS。frugiperda。检查c。floridanumrecovered fromP。includensandT。结果表明，被膜包裹的幼虫体内没有颗粒细胞，但在无膜幼虫周围形成的荚膜中存在颗粒细胞和浆细胞。这表明允许主机可能不会封装c。因为颗粒细胞不能与这个表面结合。在非宿主物种中缺乏这种保护表明，造血细胞上介导c识别的识别和/或粘附受体存在变异。floridanum。
C. floridanumfirst instar larvae isolated from laboratory-rearedPseudoplusia includens[Chrysodeixis includens] andTrichoplusia niwere incubated for 30 minutes in phosphate buffer saline (PBS; 7.2 pH) with or without dispase (1 U/100 µl). The larvae in PBS remained envelope by the membrane. After rinsing in PBS, the larvae were inoculated into permissive (P. includensandT. ni) and non-host (Spodoptera frugiperda, Heliothis virescens, andManduca sexta) species. All hosts were dissected 24 h post-inoculation, and the number of recovered larvae that were encapsulated was determined by phase-contrast microscopy. In general, the presence or absence of the syncytial membrane strongly affected the encapsulation rate in all host species. The larvae without an extraembryonic membrane were encapsulated irrespective of host species. The membrane-enveloped larvae were almost never encapsulated inP. includensorT. ni, but an increasing proportion of larvae were encapsulated in the non-host species. The larvae enveloped by a syncytial membrane elicited the weakest encapsulation response inP. includensandT. ni, an intermediate response inH. virescens, and the strongest responses inM. sextaandS. frugiperda. The inspection ofC. floridanumrecovered fromP. includensandT. niindicated that no granular cells bound to the membrane-enveloped larvae, but granular cells and plasmocytes were present in the capsules that formed around the larvae without a membrane. This suggests that permissive hosts may not encapsulateC. floridanumbecause granular cells are unable to bind to this surface. The absence of such protection in non-host species suggests that variation exist in the recognition and/or adhesion receptors on haemocytes that mediate the recognition ofC. floridanum.