Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
复制标题
DOI：
10.3389/fimmu.2021.719037
复制
发表时间：
2021
影响因子：
7.3
通讯作者：
Corbett KD
中科院分区：
文献类型：
--
作者：
Ye Q;Lu S;Corbett KD
由冠状病毒SARS-CoV-2引起的新冠肺炎大流行是21世纪最严重的公共卫生事件。虽然已经开发出针对SARS-CoV-2的有效疫苗，但仍然迫切需要诊断方法来快速准确地检测感染。抗原测试，特别是那些检测丰富的SARS-CoV-2核衣壳蛋白的测试，是一种被证明是检测活跃的SARS-CoV-2感染的方法。在这里，我们报道了三种骆驼来源的单域抗体的高分辨晶体结构，它们以高亲和力结合SARS-CoV-2核衣壳蛋白。每个抗体识别蛋白质的一个特定折叠结构域，两个抗体识别N-末端RNA结合域，一个抗体识别C-末端二聚结构域。识别RNA结合域的两种抗体既影响RNA结合亲和力，也影响核衣壳蛋白的RNA介导的相分离。这三种抗体都识别核衣壳蛋白上高度保守的表面，这表明它们可以用来开发负担得起的诊断测试，以检测所有正在循环的SARS-CoV-2变种。
The COVID-19 pandemic, caused by the coronavirus SARS-CoV-2, is the most severe public health event of the twenty-first century. While effective vaccines against SARS-CoV-2 have been developed, there remains an urgent need for diagnostics to quickly and accurately detect infections. Antigen tests, particularly those that detect the abundant SARS-CoV-2 Nucleocapsid protein, are a proven method for detecting active SARS-CoV-2 infections. Here we report high-resolution crystal structures of three llama-derived single-domain antibodies that bind the SARS-CoV-2 Nucleocapsid protein with high affinity. Each antibody recognizes a specific folded domain of the protein, with two antibodies recognizing the N-terminal RNA binding domain and one recognizing the C-terminal dimerization domain. The two antibodies that recognize the RNA binding domain affect both RNA binding affinity and RNA-mediated phase separation of the Nucleocapsid protein. All three antibodies recognize highly conserved surfaces on the Nucleocapsid protein, suggesting that they could be used to develop affordable diagnostic tests to detect all circulating SARS-CoV-2 variants.
登录
查看更多内容
DOI：
10.1107/s0907444910045749
发表时间：
2011-04
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Winn MD;Ballard CC;Cowtan KD;Dodson EJ;Emsley P;Evans PR;Keegan RM;Krissinel EB;Leslie AG;McCoy A;McNicholas SJ;Murshudov GN;Pannu NS;Potterton EA;Powell HR;Read RJ;Vagin A;Wilson KS
通讯作者：
Wilson KS
BNT162B2 mRNA COVID-19疫苗的安全性和功效。
DOI：
10.1056/nejmoa2034577
发表时间：
2020-12-31
期刊：
The New England journal of medicine
影响因子：
--
作者：
Polack FP;Thomas SJ;Kitchin N;Absalon J;Gurtman A;Lockhart S;Perez JL;Pérez Marc G;Moreira ED;Zerbini C;Bailey R;Swanson KA;Roychoudhury S;Koury K;Li P;Kalina WV;Cooper D;Frenck RW Jr;Hammitt LL;Türeci Ö;Nell H;Schaefer A;Ünal S;Tresnan DB;Mather S;Dormitzer PR;Şahin U;Jansen KU;Gruber WC;C4591001 Clinical Trial Group
通讯作者：
C4591001 Clinical Trial Group
DOI：
10.15252/embj.2020106478
发表时间：
2020-12-15
期刊：
The EMBO journal
影响因子：
--
作者：
Perdikari TM;Murthy AC;Ryan VH;Watters S;Naik MT;Fawzi NL
通讯作者：
Fawzi NL
DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group
影响因子：
8
作者：
Ye, Qiaozhen;West, Alan M. V.;Corbett, Kevin D.
通讯作者：
Corbett, Kevin D.