Stretch-activated ion channels identified in the touch-sensitive structures of carnivorous Droseraceae plants.
Stretch-activated ion channels identified in the touch-sensitive structures of carnivorous Droseraceae plants.
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DOI：
10.7554/elife.64250
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发表时间：
2021-03-16
期刊：
影响因子：
7.7
通讯作者：
Chory J
中科院分区：
文献类型：
--
作者：
Procko C;Murthy S;Keenan WT;Mousavi SAR;Dabi T;Coombs A;Procko E;Baird L;Patapoutian A;Chory J
作为对触摸的反应，一些食肉植物，如捕蝇草，进化出了壮观的动作来捕捉动物以获取营养。然而，赋予这种敏感性的分子仍然未知。研究人员利用比较转录组学方法发现，在捕蝇草的触感触发毛中，有3个编码机械敏感离子通道MscS-Like （MSL）和OSCA/TMEM63家族同源基因的表达。我们将重点放在触发毛中表达最丰富的候选基因，即MSL同源基因FLYCATCHER1 （FLYC1）。我们发现FLYC1转录本定位于触发毛内的机械感觉细胞，转染FLYC1可在naïve细胞中诱导氯离子渗透拉伸激活电流，编码FLYC1同源物的转录本在角菜（一种相关的菊科食肉植物）的触摸感应细胞中表达。我们的数据表明，肉食性果蝇科的猎物识别机制是通过吸收祖先的机械敏感离子通道来感知触觉而进化的。
In response to touch, some carnivorous plants such as the Venus flytrap have evolved spectacular movements to capture animals for nutrient acquisition. However, the molecules that confer this sensitivity remain unknown. We used comparative transcriptomics to show that expression of three genes encoding homologs of the MscS-Like (MSL) and OSCA/TMEM63 family of mechanosensitive ion channels are localized to touch-sensitive trigger hairs of Venus flytrap. We focus here on the candidate with the most enriched expression in trigger hairs, the MSL homolog FLYCATCHER1 (FLYC1). We show that FLYC1 transcripts are localized to mechanosensory cells within the trigger hair, transfecting FLYC1 induces chloride-permeable stretch-activated currents in naïve cells, and transcripts coding for FLYC1 homologs are expressed in touch-sensing cells of Cape sundew, a related carnivorous plant of the Droseraceae family. Our data suggest that the mechanism of prey recognition in carnivorous Droseraceae evolved by co-opting ancestral mechanosensitive ion channels to sense touch.