The distribution profiles of tetracycline resistance genes in rice: Comparisons using four genotypes.
The distribution profiles of tetracycline resistance genes in rice: Comparisons using four genotypes.
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DOI：
10.1016/j.scitotenv.2023.168359
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发表时间：
2023-11
期刊：
影响因子：
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通讯作者：
Yijun Kang;Sumeng Zhao;Haoyang Cheng;Wenjie Xu;Ruiqiang You;Jian Hu
中科院分区：
文献类型：
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作者：
Yijun Kang;Sumeng Zhao;Haoyang Cheng;Wenjie Xu;Ruiqiang You;Jian Hu
抗生素抗性基因（ARG）从根际到植物和人类的潜在传播引起了人们的极大关注。本研究旨在研究四环素抗性基因（TRG）在水稻中的分布，并确定水稻籽粒中TRG的主要来源。采用定量聚合酶链反应（qPCR）技术，对4个水稻品种和3个水稻分区在拔节期和抽穗期的7个TRG和intI1基因的丰度进行了分析。对细菌群落进行分析，以阐明TRG谱的潜在机制。结果表明，tetZ主要存在于根际和根内，而tetX则在籽粒中占优势。TRGs和intI1的相对丰度在品种和分区间均表现出显著的差异。然而，没有观察到显着差异，在粮食中，TRG的丰度是几个数量级低于根际相比。然而，TRG传播给人类的潜在风险，特别是谷物中潜在病原体携带的TRG，值得关注。与粳稻品种相比，杂交稻品种根际和根内TRGs积累的可能性增加，可能是由于其根系代谢活性增强。intI1和TRG之间观察到的显着关联，再加上在不同治疗中intI1潜在宿主的实质性变化，表明intI1介导的水平基因转移在TRG的各种细菌宿主中发挥作用。研究还表明，根际细菌在拔节期作为主要贡献者的TRG在籽粒中通过内根连接。研究结果表明，粳稻品种表现出上级控制TRG相比，杂交品种，强调需要在整个水稻生长期的早期干预。
The potential transmission of antibiotic resistance genes (ARGs) from the rhizosphere to plants and humans poses a significant concern. This study aims to investigate the distribution of tetracycline resistance genes (TRGs) in rice using four genotypes and identify the primary source of TRGs in grains. Quantitative polymerase chain reaction (qPCR) was employed to determine the abundance of seven TRGs andintI1in four rice varieties and three partitions during the jointing and heading stages, respectively. The analysis of the bacterial community was conducted to elucidate the underlying mechanism of the profiles of TRGs. It was observed thattetZwas predominantly present in the rhizosphere and endoroot, whereastetXbecame dominant in grains. The relative abundances of TRGs andintI1exhibited significant variations across both the variety and partition. However, no significant differences were observed in grains, where the abundances of TRGs were several orders of magnitude lower compared to those in the rhizosphere. Nevertheless, the potential risk of the dissemination of TRGs to humans, particularly those carried by potential pathogens in grains, warrants attention. The increased likelihood of TRGs accumulation in the rhizosphere and endoroot of hybrid rice varieties, as opposed to japonica varieties, may be attributed to the heightened metabolic activities of their roots. The significant associations observed betweenintI1and TRGs, coupled with the substantial alterations in potential hosts forintI1across various treatments, indicate thatintI1-mediated horizontal gene transfer plays a role in the diverse range of bacterial hosts for TRGs. The study also revealed that rhizosphere bacteria during the jointing stage serve as the primary contributors of TRGs in grains through the endoroot junction. The findings indicate that Japonica rice varieties exhibit superior control over TRGs compared to hybrid varieties, emphasizing the need for early interventions throughout the entire growth period of rice.