The development and use of Actiphage to detect viable mycobacteria from bovine tuberculosis and Johne's disease-infected animals
The development and use of Actiphage to detect viable mycobacteria from bovine tuberculosis and Johne's disease-infected animals
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DOI：
10.1111/1751-7915.13518
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发表时间：
2019-12-03
影响因子：
5.7
通讯作者：
Rees, Catherine E. D.
中科院分区：
文献类型：
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作者：
Swift, Benjamin M. C.;Meade, Nathan;Rees, Catherine E. D.
在这里，我们描述了一种利用噬菌体D29作为裂解剂从低数量的分枝杆菌细胞中高效提取DNA的方法的发展。该方法(Actiphage)与聚合酶链式反应(PCR)相结合，实现了快速、灵敏(LOD
Here, we describe the development of a method that exploits bacteriophage D29 as a lysis agent for efficient DNA extraction from low numbers of mycobacterial cells. This method (Actiphage) used in combination with PCR achieved rapid and sensitive (LOD