Survival of biological cells deformed in a narrow gap.
Survival of biological cells deformed in a narrow gap.
复制标题
生存的生物细胞在狭窄的缝隙中变形。
DOI：
10.1115/1.1516197
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
N. Kumagae
中科院分区：
文献类型：
--
作者：
H. Takamatsu;N. Kumagae
近年来的研究表明，在生物细胞的缓慢冷冻过程中，细胞不仅会受到化学损伤，还会受到冰晶压缩引起的机械损伤。提出了一种新的实验方法来量化两个平行表面的变形对细胞的破坏。在0 ℃、23 ℃和37 ℃下，测量细胞（前列腺癌细胞，平均直径为17.5微米）的存活率作为3.5微米至16.2微米范围内的间隙尺寸的函数。在较小间隙尺寸下的生存力在37 ℃下显著低于在23 ℃下，而0 ℃和23 ℃之间的差异小得多。这表明变形损伤与细胞骨架的变形有关，而不是与脂膜的机械性质有关。
Recent studies show that during slow freezing of biological cells, the cells may be also injured by not only chemical damage but also mechanical damage induced by ice crystal compression. A new experimental procedure is developed to quantify cell destruction by deformation with two parallel surfaces. The viability of cells (prostatic carcinoma cells, 17.5 microns in mean diameter) is measured as a function of gap size ranging from 3.5 microns to 16.2 microns at 0 degree C, 23 degrees C and 37 degrees C. The viability at a smaller gap size is significantly lower at 37 degrees C than at 23 degrees C, while the difference between 0 degree C and 23 degrees C is much smaller. This suggests that deformation damage is related to the deformation of the cytoskeleton rather than the mechanical properties of the lipid membrane.