Ethylenediamine-assisted hydrothermal synthesis of nitrogen-doped carbon quantum dots as fluorescent probes for sensitive biosensing and bioimaging
Ethylenediamine-assisted hydrothermal synthesis of nitrogen-doped carbon quantum dots as fluorescent probes for sensitive biosensing and bioimaging
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DOI：
10.1016/j.snb.2015.05.006
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发表时间：
2015-10-31
影响因子：
8.4
通讯作者：
Zhang, Xue-Ji
中科院分区：
文献类型：
--
作者：
Niu, Wen-Jun;Li, Ye;Zhang, Xue-Ji
本文以丙氨酸为单一碳源，乙二胺为表面钝化剂，采用一锅水热碳化法成功合成了高荧光量子产率（46.2%）的氮掺杂碳量子点（N-CQD）。所制备的N-CQDs具有良好的生物相容性，可有效地标记MCF-7细胞膜和细胞核，在生物标记和生物成像方面具有潜在的应用价值。此外，添加二氢烟酰胺腺嘌呤二核苷酸（NADH）可以显着淬灭N-CQDs的荧光。因此，建立了一种新的测定NADH的方法，并对其机理进行了探讨。该荧光传感器的线性范围可达80 μ M，检测限可达25.1 nM，对NADH的检测性能有明显的提高。(C)2015 Elsevier B. V.版权所有。
In this work, nitrogen-doped carbon quantum dots (N-CQDs) with high fluorescence quantum yield (46.2%) were successfully synthesized through one-pot hydrothermal carbonization approach assisted by ethylenediamine as surface passivation agent, wherein alanine acted as a single carbon source, ethylenediamine as the surface passivation agent. The obtained biocompatible N-CQDs could effectively label both the MCF-7 cell membranes and the nucleus, suggesting the potential application in biolabeling and bioimaging. Furthermore, the addition of dihydronicotinamide adenine dinucleotide (NADH) could dramatically quench the fluorescence of N-CQDs. Therefore, a novel protocol for NADH determination was established and its mechanism was discussed. This proposed fluorescence sensor displayed an enhanced performance for NADH determination with the linear range up to 80 mu M and the detection limit down to 25.1 nM. (C) 2015 Elsevier B.V. All rights reserved.