EFFECTS OF HEAVY-METAL IONS ON SELECTED OXIDATIVE METABOLIC PROCESSES IN RAT ALVEOLAR MACROPHAGES
EFFECTS OF HEAVY-METAL IONS ON SELECTED OXIDATIVE METABOLIC PROCESSES IN RAT ALVEOLAR MACROPHAGES
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DOI：
10.1016/0041-008x(80)90375-0
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发表时间：
1980-01-01
影响因子：
3.8
通讯作者：
MILES, PR
中科院分区：
文献类型：
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作者：
CASTRANOVA, V;BOWMAN, L;MILES, PR
研究了4种重金属阳离子（Cd ~（2+）、Hg ~（2+）、Ni ~（2+）和Pb ~（2+））对大鼠肺泡巨噬细胞在静息状态（无吞噬作用）和吞噬过程中氧消耗、葡萄糖代谢和活性氧释放（化学发光法测定）的影响。所有4种重金属抑制O2消耗和葡萄糖代谢，这发生在肺泡巨噬细胞在休息约60- 95%。在吞噬过程中，有释放的反应形式的O2消耗，但没有改变葡萄糖代谢发生在静息细胞。这些金属抑制活性O2从细胞中的释放和在吞噬过程中发生的O2消耗75- 85%。ED 50值，即，产生最大效应的1/2的金属浓度表明，活性O2释放机制受到的金属浓度比O2消耗量低得多的影响。台盼蓝实验提供的证据表明，金属可以影响氧化代谢，而不会造成严重的膜损伤。重金属明显抑制肺泡巨噬细胞的氧化代谢过程，并可能降低这些细胞的抗菌活性。
The effects of 4 heavy metal cations (Cd2+, Hg2+, Ni2+, and Pb2+) on O2 consumption, glucose metabolism, and the release of active O2 species (as measured by chemiluminescence) were studied in rat alveolar macrophages at rest (no phagocytosis) and during phagocytosis. All 4 heavy metals depress the O2 consumption and glucose metabolism which occurs in alveolar macrophages at rest by about 60-95%. During phagocytosis there is release of reactive forms of O2 consumption, but no change in glucose metabolism from that which occurs in resting cells. The metals inhibit the release of active O2 from the cells and the O2 consumption which occurs during phagocytosis by 75-85%. The ED50 values, i.e., the concentrations of metals which produce 1/2 of the maximal effects, indicate that the mechanism for release of active O2 is affected by much lower concentrations of metals than is O2 consumption. Experiments with trypan blue provide evidence that the metals can affect oxidative metabolism without causing gross membrane damage. Heavy metals evidently inhibit oxidative metabolic processes in alveolar macrophages and may diminish the antibacterial activity of these cells.