Synthesis and Characterization of a Laminated Hydroxyapatite/Gelatin Nanocomposite Scaffold with Controlled Pore Structure for Bone Tissue Engineering
Synthesis and Characterization of a Laminated Hydroxyapatite/Gelatin Nanocomposite Scaffold with Controlled Pore Structure for Bone Tissue Engineering
复制标题
用于骨组织工程的具有受控孔隙结构的层状羟基磷灰石/明胶纳米复合支架的合成和表征
DOI：
10.1177/039139881003300204
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
S. A. Poursamar
中科院分区：
文献类型：
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作者：
M. Azami;Ali Samadikuchaksaraei;S. A. Poursamar
本研究以羟基磷灰石（HA）和明胶（GEL）为主要成分，设计了一种用于骨修复的纳米结构支架。合成了HA纳米粉末，并与GEL一起用于设计结合层溶剂浇铸、冷冻干燥和层压3种技术的三维纳米复合材料。结果表明，该支架具有三维连通的均匀多孔结构，孔隙率为82%，孔径为300 ~ 500 μm。它也已被证明，机械指标的范围内的海绵骨。培养的成骨细胞样细胞（SaOS-2）显示出良好的细胞附着，迁移和渗透到纳米复合材料支架的孔隙中。在这里，我们已经表明，通过广泛使用的方法与简单的实验操作相结合，纳米HA粉末可以合成和用于制造任何所需形状的三维HA/GEL纳米复合材料，其机械性能与松质骨相当。
In this study, a nanostructured scaffold was designed for bone repair using hydroxyapatite (HA) and gelatin (GEL) as its main components. Nanopowders of HA were synthesized, and together with GEL, used to engineer a 3-dimensional nanocomposite combining 3 techniques of layer solvent casting, freeze-drying, and lamination. The results show that the scaffold possesses a 3-dimensional interconnected homogenous porous structure with a porosity of 82% and pore sizes ranging from 300 to 500 μm. It has also been shown that mechanical indices are in the range of spongy bones. Cultured osteoblast-like cells (SaOS-2) have shown an excellent level of cell attachment, migration, and penetration into the porosities of the nanocomposite scaffold. Here, we have shown that by a combination of widely available methods with simple experimental operations, nano-HA powders can be synthesized and used to make 3-dimensional HA/GEL nanocomposites in any desired shape, with mechanical properties comparable to spongy bone.