Improving phenotyping in winter barley cultivars towards waterlogging tolerance by combining field trials under natural conditions with controlled growth condition experiments
Improving phenotyping in winter barley cultivars towards waterlogging tolerance by combining field trials under natural conditions with controlled growth condition experiments
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DOI：
10.1016/j.eja.2021.126432
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发表时间：
2021-12-02
影响因子：
5.2
通讯作者：
Barth, Susanne
中科院分区：
文献类型：
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作者：
Byrne, Tomas;Grant, James;Barth, Susanne
由于全球气候变化，北方的降雨量增加，导致田间洪水的发生率增加。洪水造成缺氧压力，导致光合作用能力降低，养分供应和吸收减少，土壤中厌氧细菌产生的有毒代谢物增加，最终导致产量损失和作物死亡。为了克服低氧环境条件，需要培育新品种并进行耐涝性测试。我们从“英国精英大麦协会遗传学”（AGOUEB）群体中获得了403个冬大麦品种，利用与低氧胁迫相关的表型变化。这使我们能够确定一个初始的一组涝渍敏感和耐受品种。一个子集的65个品种暴露于内涝胁迫下的领域和生长柜环境的比较分析表明，由于不同的敏感性，多季节的田间试验内涝得分的变化。在田间试验中，我们观察到水涝损害导致生物量，粮食产量和作物高度的减少。然而，由于农田地形和降雨量的差异，不同季节和不同的内涝严重程度造成的影响各不相同。为了克服多季田间试验中环境条件的季节性变化，我们平行开发了一种增强的表型分析方法，该方法通过在受控生长条件下进行表型分析来补充田间试验。表型评分方法允许通过对涝渍的敏感性和耐受性对品种进行分组，在田间和受控条件下评分的品种之间的差异有限。总之，这两种互补的方法最大限度地提高了育种者可用的数据，允许可靠地选择能够在洪水条件下生长的更耐受的品种。
Additional rainfall in Northern Europe due to global climate change is increasing the incidences of field flooding. Flooding causes hypoxic stress that results in a reduced capacity for photosynthesis, reduction in nutrient availability and uptake, increased production of toxic metabolites by anaerobic bacteria in the soil, and ultimately yield losses and crop death. To overcome hypoxic environmental conditions, new cultivars need to be bred and tested for waterlogging tolerance. We scored 403 winter barley cultivars from the 'Association Genetics of UK Elite Barley' (AGOUEB) population, taking advantage of the phenotypic changes associated with hypoxic stress. This enabled us to identify an initial set of waterlogging sensitive and tolerant cultivars. Comparative analysis of a subset of 65 cultivars exposed to waterlogging stress under field and growth cabinet environments showed variability in scores due to varying sensitivity to waterlogging over multi-season field trials. In field trials, we observed waterlogging damage resulting in reductions in biomass, grain yield and crop height. However, the effects varied between seasons and the severity of waterlogging due to differences in the topography of the field and the amount of rainfall. To overcome the seasonal variations in environmental conditions in multi-season field trials, we developed in parallel, an enhanced phenotyping method by complementing field experiments with phenotyping under controlled growth conditions. The phenotyping scoring method allows for the grouping of cultivars by sensitivity and tolerance to waterlogging, with limited variance between cultivars scored in the field and controlled conditions. Together, these two complementary approaches maximise the data available to breeders, allowing for the reliable selection of more tolerant cultivars able to grow under flooding conditions.