Proton pump OsA8 is linked to phosphorus uptake and translocation in rice
Proton pump OsA8 is linked to phosphorus uptake and translocation in rice
复制标题
质子泵 OsA8 与水稻磷的吸收和转运有关
DOI：
10.1093/jxb/ern298
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发表时间：
2009-02-01
影响因子：
6.9
通讯作者：
Xu, Guohua
中科院分区：
文献类型：
--
作者：
Chang, Chunrong;Hu, Yibing;Xu, Guohua
质膜 (PM) 质子泵 ATP 酶 (H+-ATP 酶) 几乎涉及生物学的所有方面。它们是植物特有的，这个家族的几个成员应该在营养获取中发挥关键作用。目前，仅对植物中该基因家族的一些成员进行了表征。然而，尚未对水稻中与营养吸收相关的 H+-ATPase 基因进行功能分析。据报道，OsA8 是一种典型的 PM H+-ATP 酶基因，主要在水稻根部表达，因营养缺乏而下调。在磷（P）饥饿条件下，Osa8突变体具有相对较小的尺寸和根茎生物量比，但比其野生型对应物具有更高的ATP酶活性。 OsA8 的敲除影响了几个 OsA 基因和高亲和力磷酸盐转运蛋白 OsPT6 的表达，并导致根中磷浓度较高，芽中磷含量较低。这些分析表明，OsA8 不仅影响水稻根部对磷的吸收，还影响水稻中磷从根部到地上部的转运。
The plasma membrane (PM) proton pump ATPases (H+-ATPases) are involved in almost all aspects of biology. They are plant specific and several members of this family are supposed to play a key role in nutrient acquisition. At present, only some members of this gene family in plants have been characterized. However, no nutrient uptake associated H+-ATPase gene in rice has been functionally analysed. It is reported here that OsA8, a typical PM H+-ATPases gene that was predominantly expressed in roots of rice, is down-regulated by nutrient deficiency. The Osa8 mutant had a relatively smaller size and root to shoot biomass ratio, but higher ATPase activity than its wild-type counterparts under phosphorus (P) starvation conditions. Knockout of OsA8 affected the expression of several OsA genes and the high affinity phosphate transporter, OsPT6, and resulted in a higher P concentration in the roots and a lower amount of P in the shoots. These analyses demonstrate that OsA8 not only influences the uptake of P by roots, but also the translocation of P from the roots to the shoots in rice.