RESTRICTION OF LAMBDA-TRP BACTERIOPHAGES BY ESCHERICHIA-COLI K
RESTRICTION OF LAMBDA-TRP BACTERIOPHAGES BY ESCHERICHIA-COLI K
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DOI：
10.1016/0022-2836(74)90529-4
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发表时间：
1974-01-01
影响因子：
5.6
通讯作者：
WINTON, S
中科院分区：
文献类型：
--
作者：
BRAMMAR, WJ;MURRAY, NE;WINTON, S
trp-transducing derivatives of phage λ have been used to studyEscherichia coliK specific restrictionin vivo. The expression of thetrpgenes from unmodified phages during infection of arec+, restricting host is eliminated by restriction. In a K-restrictingrecB,Chost, where degradation of restricted phage DNA is prevented, expression of thetrpgenes is little affected by the presence of a single unmodified, K-restriction recognition site, even when that site is within thetrpEgene. RI restriction, in contrast to K restriction, preventstrpgene expression in arecB,Chost when the restriction target is between thetrpgenes and the relevant promoter. The presence oftwoK-restriction recognition sites in a λtrpphage can have a marked effect ontrpgene expression. This effect can be interpreted as the result of preferential breakage between the two restriction recognition sites. We conclude that K restriction does not break susceptible DNA at, or even preferentially near, a restriction recognition sequence.
