MALDI-TOF and MALDI-FTICR imaging mass spectrometry of methamphetamine incorporated into hair
MALDI-TOF and MALDI-FTICR imaging mass spectrometry of methamphetamine incorporated into hair
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DOI：
10.1002/jms.1908
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发表时间：
2011-04-01
影响因子：
2.3
通讯作者：
Suzuki, Koichi
中科院分区：
文献类型：
--
作者：
Miki, Akihiro;Katagi, Munehiro;Suzuki, Koichi
介绍了一种利用基质辅助激光解吸电离(MALDI)飞行时间(TOF)和MALDI傅里叶变换离子回旋共振(FTICR)对人发中甲基苯丙胺(MA)进行成像质谱分析的新方法。用α-氰基-4-羟基肉桂酸基质沉积后，用MALDI-TOF-IMS直接分析了一例慢性MA使用者的纵向手工切割的单个人发干的纵向切片。通过在所选择的反应监控模式中监控MA特定的产物离子，首次获得了最可能是通过重复摄入MA而产生的条形码状图像。激光扫描纵向切割的发干只能得到较差的MA质谱图，这可能是由于MA的丢失和/或头发的热变性。用MALDI-FTICR质谱仪进一步证实了头发中MA的真实性。与FTICR的超高分辨率质谱学相结合，提供了无可争议的MA鉴定。同一MA用户的另一根发干的MALDI-FTICR-IMS也通过以超高分辨率监测MA质子化分子而提供条形码般的图像。这两个条形码的图像显示出非常相似的地方。因此，MALDI-IMS可以提供一个新的视角：“为药物的毛发分析成像”。版权所有(C)2011 John Wiley&Sons，Ltd.
A new approach is described for imaging mass spectrometry (IMS) of methamphetamine (MA) incorporated into human hair using matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF) and MALDI-Fourier transform ion cyclotron resonance (FTICR). A longitudinal section of a lengthwise manually-cut single human hair shaft from a chronic MA user was directly analyzed by MALDI-TOF-IMS after deposited with alpha-Cyano-4-hydroxycinnamic acid matrix. A barcode-like image, which was most probably generated with repeated intakes of MA, was for the first time obtained by monitoring MA-specific product ion in the selected reaction monitoring mode. Laser beam scan lengthwise-cut hair shafts gave only poor mass spectra of MA, probably due to the loss of MA and/or the thermal denaturation of hair. The identity of MA detected in hair was further confirmed by MALDI-FTICR mass spectrometry. A combination with ultra-high resolution mass spectrometry by FTICR provided indisputable identification of MA. The MALDI-FTICR-IMS of another hair shaft from the same MA user also provided a barcode-like image by monitoring the protonated molecule of MA with ultra-high resolution. The two barcode-like images exhibited a close resemblance. Thus, MALDI-IMS can offer a new perspective: 'imaging hair analyses for drugs'. Copyright (C) 2011 John Wiley & Sons, Ltd.