Loss of Calmodulin Binding to Bax Inhibitor-1 Affects Pseudomonas-mediated Hypersensitive Response-associated Cell Death in Arabidopsis thaliana*
Loss of Calmodulin Binding to Bax Inhibitor-1 Affects Pseudomonas-mediated Hypersensitive Response-associated Cell Death in Arabidopsis thaliana*
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DOI：
10.1074/jbc.m109.037234
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发表时间：
2009-08
期刊：
影响因子：
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通讯作者：
M. Kawai‐Yamada;Zenta Hori;Taro Ogawa;Yuri Ihara-Ohori;K. Tamura;M. Nagano;T. Ishikawa;H. Uchimiya-H.-U
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文献类型：
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作者：
M. Kawai‐Yamada;Zenta Hori;Taro Ogawa;Yuri Ihara-Ohori;K. Tamura;M. Nagano;T. Ishikawa;H. Uchimiya-H.-U
Bax 抑制剂-1 (BI-1) 是一种在多种生物体中保守的细胞死亡抑制蛋白。拟南芥atbi1-1植物是一种突变体，其中表达的BI-1蛋白的C端6个氨基酸已被T-DNA插入取代。这种大肠杆菌产生的突变 BI-1 蛋白 (AtBI-CM) 不再能与钙调蛋白结合。启动子-报告基因检测证明了 BI-1 在过敏反应期间的区室化表达，这是由接种具有 avrRTP2 基因 Pst(avrRPT2) 的丁香假单胞菌引起的。此外，BI-1 敲低植物和 atbi1-1 均表现出对 Pst(avrRPT2) 诱导的细胞死亡的敏感性增加。结果表明，钙调蛋白结合的丧失降低了植物中 BI-1 的细胞死亡抑制活性。
Bax inhibitor-1 (BI-1) is a cell death suppressor protein conserved across a variety of organisms. The Arabidopsis atbi1-1 plant is a mutant in which the C-terminal 6 amino acids of the expressed BI-1 protein have been replaced by T-DNA insertion. This mutant BI-1 protein (AtBI-CM) produced in Escherichia coli can no longer bind to calmodulin. A promoter-reporter assay demonstrated compartmentalized expression of BI-1 during hypersensitive response, introduced by the inoculation of Pseudomonas syringae possessing the avrRTP2 gene, Pst(avrRPT2). In addition, both BI-1 knockdown plants and atbi1-1 showed increased sensitivity to Pst(avrRPT2)-induced cell death. The results indicated that the loss of calmodulin binding reduces the cell death suppressor activity of BI-1 in planta.