Basis of the persistence of capsule-negative Streptococcus suis in porcine endocarditis inferred from comparative genomics
Basis of the persistence of capsule-negative Streptococcus suis in porcine endocarditis inferred from comparative genomics
复制标题
从比较基因组学推断猪心内膜炎中荚膜阴性猪链球菌持续存在的基础
DOI：
10.1093/femsle/fnab083
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发表时间：
2021
影响因子：
2.1
通讯作者：
Sekizaki Tsutomu
中科院分区：
文献类型：
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作者：
Tohya Mari;Dozaki Shinichi;Ishida-Kuroki Kasumi;Watanabe Takayasu;Sekizaki Tsutomu
猪链球菌的荚膜是一种抗吞噬因子，是其主要毒力因子之一。然而，我们在猪心内膜炎中发现了帽阳性和帽阴性分离株。在这里，我们比较了多重冠状病毒阴性分离株与冠状病毒阳性分离株的基因组序列。来自同一头猪的CAP阳性和CAP阴性分离株与来自其他猪的分离株相比在遗传学上最接近。部分来自同一头猪的cap阴性分离株在荚膜多糖合成（cps）基因上出现不同的突变，表明这些分离株是在感染后从猪体内产生的。全基因组中的突变也在具有相同突变的分离物中发现，表明全基因组和基因组中的突变独立发生。由于冠状病毒阴性分离株很少在其他疾病的病变中发现，这些结果表明，心内膜炎病变可能只是有利于冠状病毒阴性突变体生存的小生境，导致他们在病变的持久性。
The capsule (cap) ofStreptococcus suisis an anti-phagocytic element and is one of the major virulence factors. However, we have found cap-positive and cap-negative isolates in porcine endocarditis. Here, we compared genome sequences of multiple cap-negative isolates with those of a cap-positive isolate from a single endocarditis. Cap-positive and cap-negative isolates from the same pig were phylogenetically closest compared with those from other pigs. Some of cap-negative isolates from the same pig showed different mutations in capsular polysaccharide synthesis (cps) genes, suggesting that these isolates arisen in pigs after infection. Different mutations in whole-genomes were also found among isolates with identical mutations incpsgenes, indicating that mutations incpsgenes and the whole-genome occurred independently. Since cap-negative isolates are rarely found in lesions of other diseases, these results suggest that endocarditis lesions may simply favored cap-negative mutants to survive the niches, leading to their persistence in the lesions.