In vivo and in vitro 31P-NMR preliminary studies of the VX-2 carcinoma in rabbits.
In vivo and in vitro 31P-NMR preliminary studies of the VX-2 carcinoma in rabbits.
复制标题
兔VX-2癌体内外31P-NMR初步研究。
DOI：
10.1097/00004424-198808000-00008
复制
发表时间：
1988
影响因子：
6.7
通讯作者：
Anderson,JH
中科院分区：
文献类型：
--
作者：
Mills,P;Buonomo,C;Pillai,RP;SamphilipoJr,MA;Rajan,SS;Strandberg,JD;Anderson,JH
采用体内和体外 31 P-NMR 波谱研究了植入兔肝、肾和后肢肌肉的 VX-2 肿瘤的高能磷酸盐代谢。首先通过体内 31 P-NMR 光谱检查处于不同生长阶段的肿瘤，然后将其切除并进行组织学检查和生化分析；使用体外 31 P-NMR 和标准酶技术。体内NMR谱、体外NMR数据和生化分析之间具有良好的相关性。尽管肿瘤光谱显示出与其他肿瘤模型中报道的特征相似的特征，但从植入同一部位和不同部位的肿瘤获得的光谱存在显着的差异。肿瘤坏死程度与肿瘤pH、Pi:ATP比值与坏死的相关性较差。这种变异性对于使用 31 P-NMR 光谱监测体内肿瘤生长和治疗的潜在价值具有重要意义。
In vivo and in vitro 31 P-NMR spectroscopy was used to study the high energy phosphate metabolism of VX-2 tumors implanted into rabbit liver, kidney, and hind-limb muscle. Tumors, at various stages of growth, were first examined by in vivo 31 P-NMR spectroscopy, then they were excised and underwent histologic examination and biochemical analysis; both in vitro 31 P-NMR and standard enzymatic techniques were used. There was good correlation among the in vivo NMR spectra, the in vitro NMR data, and the biochemical analyses. Although the tumor spectra showed characteristics similar to those reported in other tumor models, there was a striking variability in the spectra obtained from tumors implanted in the same site and from different sites. There was poor correlation between the degree of necrosis in the tumor and the tumor pH and between the Pi: ATP ratio and necrosis. This variability has important implications for the potential value of using 31 P-NMR spectroscopy to monitor tumor growth and therapy in vivo.