Hormonal Signaling Cascade during an Early-Adult Critical Period Required for Courtship Memory Retention in Drosophila
Hormonal Signaling Cascade during an Early-Adult Critical Period Required for Courtship Memory Retention in Drosophila
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DOI：
10.1016/j.cub.2017.08.017
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发表时间：
2017-09-25
期刊：
影响因子：
9.2
通讯作者：
Adams, Michael E.
中科院分区：
文献类型：
--
作者：
Lee, Sang Soo;Ding, Yike;Adams, Michael E.
记忆的形成和表达对于上下文相关的决策至关重要。在果蝇中，一只求偶的雄果蝇被一只交配的雌果蝇拒绝后，当它与一只处女雌果蝇配对时，求偶的热情就降低了，这是一种归因于“求偶记忆”的行为改变。在这里，我们展示了荷尔蒙状态对维持求爱记忆的关键作用。蜕皮触发激素（ETH）通过调节成年雄性的保幼激素（JH）水平而对求偶记忆至关重要。通过ETH信号传导基因沉默降低JH水平会损害短期求爱记忆，这是一种可通过JH类似物甲氧普烯挽救的表型。JH缺陷引起的记忆障碍涉及快速衰退，而不是记忆获取失败。一个关键时期支配着成年期前3天的记忆表现。使用表达性肽的“伪交配”训练者，我们发现在没有令人厌恶的化学交配线索的情况下引发的强烈的求爱记忆也依赖于ETH-JH信号。最后，我们发现，JH通过多巴胺能神经元的行为，并得出结论，ETH-JH多巴胺信号级联需要在一个关键时期促进社会背景依赖的记忆。
Formation and expression of memories are critical for context-dependent decision making. In Drosophila, a courting male rejected by a mated female subsequently courts less avidly when paired with a virgin female, a behavioral modification attributed to "courtship memory.'' Here we show the critical role of hormonal state for maintenance of courtship memory. Ecdysis-triggering hormone (ETH) is essential for courtship memory through regulation of juvenile hormone (JH) levels in adult males. Reduction of JH levels via silencing of ETH signaling genes impairs short-term courtship memory, a phenotype rescuable by the JH analog methoprene. JH-deficit-induced memory impairment involves rapid decay rather than failure of memory acquisition. A critical period governs memory performance during the first 3 days of adulthood. Using sex-peptide-expressing "pseudo-mated'' trainers, we find that robust courtship memory elicited in the absence of aversive chemical mating cues also is dependent on ETH-JH signaling. Finally, we find that JH acts through dopaminergic neurons and conclude that an ETH-JH dopamine signaling cascade is required during a critical period for promotion of social-context dependent memory.