The short- and long-term effects of orally administered high-dose reduced graphene oxide nanosheets on mouse behaviors
The short- and long-term effects of orally administered high-dose reduced graphene oxide nanosheets on mouse behaviors
复制标题
口服高剂量还原氧化石墨烯纳米片对小鼠行为的短期和长期影响
DOI：
10.1016/j.biomaterials.2015.07.060
复制
发表时间：
2015-11-01
期刊：
影响因子：
14
通讯作者：
Xu, Rujiao
中科院分区：
文献类型：
--
作者：
Zhang, Ding;Zhang, Zheyu;Xu, Rujiao
还原氧化石墨烯（rGO）是一种碳基纳米材料，在生物医学研究中具有巨大的潜力，包括体内癌症治疗。对毒性的担忧仍然突出，必须在临床应用前进行调查。rGO暴露对动物行为的影响，如学习和记忆能力，尚未阐明。在此，我们探讨了口服rGO对小鼠行为的短期和长期影响，包括一般自发活动水平，平衡和神经肌肉协调，探索和焦虑行为，以及使用旷场，旋转棒和Morris水迷宫测试的学习和记忆能力。与给予缓冲液分散的小鼠食物或单独的缓冲液的小鼠相比，接受高剂量的小或大rGO纳米片的小鼠在探索、焦虑样或学习和记忆行为方面显示出很小的变化，尽管一般运动活动、平衡和神经肌肉协调最初受到影响，并对rGO暴露对小鼠血清超氧化物歧化酶活性的影响等机制进行了讨论。这项工作中提出的结果旨在深入了解rGO对动物的体内毒性，特别是其对学习和记忆以及其他行为的影响。(C)2015爱思唯尔有限公司版权所有。
Reduced graphene oxide (rGO), a carbon-based nanomaterial, has enormous potential in biomedical research, including in vivo cancer therapeutics. Concerns over the toxicity remain outstanding and must be investigated before clinical application. The effect of rGO exposure on animal behaviors, such as learning and memory abilities, has not been clarified. Herein, we explored the short- and long-term effects of orally administered rGO on mouse behaviors, including general locomotor activity level, balance and neuromuscular coordination, exploratory and anxiety behaviors, and learning and memory abilities using open-field, rotarod, and Morris water maze tests. Compared with mice administered buffer-dispersed mouse chow or buffer alone, mice receiving a high dose of small or large rGO nanosheets showed little change in exploratory, anxiety-like, or learning and memory behaviors, although general locomotor activity, balance, and neuromuscular coordination were initially affected, which the mechanisms (e.g. the influence of rGO exposure on the activity of superoxide dismutase in mouse serum) were discussed. The results presented in this work look to provide a deep understanding of the in vivo toxicity of rGO to animals, especially its effect on learning and memory and other behaviors. (C) 2015 Elsevier Ltd. All rights reserved.