Variable-Pitch Dispensing Workstation and Its Application to the Preparation of Microsensor Arrays
Variable-Pitch Dispensing Workstation and Its Application to the Preparation of Microsensor Arrays
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DOI：
10.2116/analsci.24.817
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发表时间：
2008-06
影响因子：
1.6
通讯作者：
H. Aoki;T. Ikeda;M. Torimura;H. Tao
中科院分区：
文献类型：
--
作者：
H. Aoki;T. Ikeda;M. Torimura;H. Tao
我们已经开发了一个8通道的毛细管为基础的点胶工作站与可变毛细管间距机制。毛细管间距可以从1 mm到9 mm变化，以任意点胶间距同时点胶不同的溶液，允许从微孔板直接点胶到集成分析系统。为了评估其点胶性能的精度，将罗丹明G染料液滴点胶到载玻片上，并分析光学体积的值。当分配20 nL时，分配体积的误差证明为0.54 nL。在分配小体积时，发现该工作站的体积误差比在传统分配器中看到的小约100倍。即使是含有50%甘油的高粘度溶液也可以精确分配。还实现了快速分配。此外，该工作站还用于制备可寻址的8 × 12微传感器阵列芯片，提供了一种独立的、可重复的点阵列。
We have developed an 8-ch capillary-based dispensing workstation with a variable capillary pitch mechanism. The capillary intervals can be varied from 1 to 9 mm to dispense different solutions simultaneously at an arbitrary dispensing pitch, allowing direct dispensing from microplates to integrated analytical systems. To evaluate the precision of its dispensing performance, droplets of Rhodamine G dye were dispensed onto glass slides and the values of the optical volume were analyzed. The error in the dispensed volume proved to be 0.54 nL when dispensing 20 nL. In dispensing small volumes, the volume error for this workstation was found to be about 100-fold less than that seen in conventional dispensers. Even highly viscous solutions containing 50% glycerol could be dispensed with precision. Rapid dispensing was also achieved. Moreover, the application of the workstation to preparing addressable 8 × 12 microsensor array chips was demonstrated, providing an independent and reproducible spot array.