Follicular Dendritic Cell Isolation and Loading of Immune Complexes.
Follicular Dendritic Cell Isolation and Loading of Immune Complexes.
复制标题
滤泡树突状细胞的分离和免疫复合物的加载。
DOI：
10.1007/978-1-4939-7095-7_9
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发表时间：
2017
影响因子：
--
通讯作者：
M. Carroll
中科院分区：
文献类型：
--
作者：
B. Heesters;C. E. van der Poel;M. Carroll
滤泡树突状细胞（Follicular dendritic cells，FDC）是位于淋巴结和其它淋巴器官（如脾）的B细胞滤泡中心的基质细胞。由于其相对较低的丰度和难以分离，FDCs在很大程度上仍然是一个谜。在这里，我们描述了如何分离FDCs用于离体细胞培养，通过流式细胞术分选，以及如何在体内或体外用免疫复合物加载它们。
Follicular dendritic cells (FDCs) are stromal cells that are centrally located within B cell follicles of lymph nodes and other lymphoid organs such as the spleen. Due to their relative low abundance and difficulty to isolate, FDCs are still largely an enigma. Here we describe how to isolate FDCs for ex vivo cell culture, sorting by flow cytometry and how to load them in vivo or in vitro with immune complexes.
影响因子：
64.5
作者：
Krautler NJ;Kana V;Kranich J;Tian Y;Perera D;Lemm D;Schwarz P;Armulik A;Browning JL;Tallquist M;Buch T;Oliveira-Martins JB;Zhu C;Hermann M;Wagner U;Brink R;Heikenwalder M;Aguzzi A
通讯作者：
Aguzzi A