Utility of thin-layer preparations in the endometrial cytology: Evaluation of benign endometrial lesions
Utility of thin-layer preparations in the endometrial cytology: Evaluation of benign endometrial lesions
复制标题
DOI：
10.1016/j.anndiagpath.2007.05.005
复制
发表时间：
2008-04-01
影响因子：
2
通讯作者：
Ohno, Eiji
中科院分区：
文献类型：
--
作者：
Norimatsu, Yoshiaki;Kouda, Hiromi;Ohno, Eiji
本研究的目的是比较薄层细胞学（TLC）制剂与传统细胞学制剂（CCP）在正常子宫内膜（增殖性、分泌性、萎缩性）和子宫内膜腺体和间质破裂（EGBD）中的使用情况。在 6 个月的时间里，我们通过使用 Uterobrush 直接采集子宫内膜样本，整理了 158 个病例。材料包括增殖期子宫内膜40例、分泌期子宫内膜42例、萎缩性子宫内膜46例、EGBD 30例。研究了以下几点：(1)子宫内膜上皮细胞团的数量； (2)上皮细胞团块数量上存在TLC>CCP病例； (3)浓缩基质细胞簇的数量； (4) 基质细胞致密簇数量上存在 TLC > CCP 病例； (5) 存在化生团块，其中含有不规则突起的致密基质簇； (6) 有清晰的背景； (7) TLC中存在血管； (8)TLC中存在长度超过20倍物体玻璃视野直径的血管。 (1) 在所有阶段中，TLC 制剂每单位面积的上皮细胞团块数量均大于 CCP。 (2)在TLC中观察到对诊断EGBD有用且足够数量的细胞(间质细胞的致密簇和具有不规则突起的致密间质簇的化生团块)。 (3) 在所有阶段中，TLC 在清晰背景的出现上均显着高于 CCP。 (4)在物体×20眼镜中长度超过视野直径的血管的出现方面，增殖性子宫内膜和分泌性子宫内膜分别与萎缩性子宫内膜和EGBD相比非常显着。尽管TLC的制备面积比CCP小，但制备物背景干净，因此可以准确报告患者的病情。因此，TLC 制备对于正常子宫内膜期和 EGBD 的准确可靠诊断也很有用，因为制备区域有限且易于识别靶细胞团块。 (c) 2008 Elsevier Inc. 保留所有权利。
The purpose of the current study was to examine the use of thin-layer cytologic (TLC) preparation compared to conventional cytologic preparation (CCP) in the normal endometrium (proliferative, secretory, atrophic) and endometrial glandular and stromal breakdown (EGBD). During a 6-month period, we compiled 158 cases by collecting a direct endometrial sample using the Uterobrush. The material comprised 40 cases of proliferative endometrium, 42 cases of secretory endometrium, 46 cases of atrophic endometrium, and 30 cases of EGBD. The following points were investigated: (1) number of endometrial epithelial cell clumps; (2) presence of TLC > CCP cases on number of epithelial cell clumps; (3) number of condensed cluster of stromal cells; (4) presence of TLC > CCP cases on number of condensed cluster of stromal cells; (5) presence of metaplastic clumps with irregular protrusion-containing condensed stromal cluster; (6) presence of a clear background; (7) presence of blood vessel in TLC; (8) presence of blood vessel of length more than diameter of a field in object x20 glasses in TLC. (1) In all phases, the number of epithelial cell clumps per a unit area of a preparation of TLC is greater than in CCP. (2) Cells (condensed cluster of stromal cells and metaplastic clumps with irregular protrusion-containing condensed stromal cluster) of useful and adequate numbers for a diagnosis of EGBD were observed in TLC. (3) In all phases, TLC was significantly higher than CCP on the appearance of a clear background. (4) The proliferative endometrium and secretory endometrium were highly significant in comparison with atrophic endometrium and EGBD, respectively, in terms of the occurrence of a blood vessel of length more than diameter of a field in object x20 glasses. Although the preparation area of TLC is smaller than that of CCP, the preparation has a clean background so that an accurate report on the patient's condition is possible. Therefore, TLC preparation is a useful too] for the accurate and reliable diagnosis of normal endometrial phase and EGBD, because the preparation area is confined and identification of the target cell clumps is easy. (c) 2008 Elsevier Inc. All rights reserved.