Fe-56 irradiation-induced cognitive deficits through oxidative stress in mice
Fe-56 irradiation-induced cognitive deficits through oxidative stress in mice
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Fe-56 辐射通过氧化应激诱导小鼠认知缺陷
DOI：
10.1039/c6tx00282j
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发表时间：
2016
影响因子：
2.1
通讯作者：
Zhang Hong
中科院分区：
文献类型：
--
作者：
Yan Jiawei;Liu Yang;Zhao Qiuyue;Mao Aihong;Li Hongyan;Di Cuixia;Zhang Hong
随着载人航天的快速发展，人们越来越关注航天飞行的健康风险，迫切需要对航天飞行进行健康评估。宇宙环境复杂，充满辐射。由于其强烈的生物效应，56 Fe等重离子被认为是这些致命的银河射线的重要组成部分。鉴于脑功能对航天员的重要性，本研究探讨了56 Fe离子辐射对小鼠海马的长期影响及其可能机制。在我们的研究中，辐射剂量为0.5戈伊、1戈伊或2戈伊。56 Fe离子全身染毒1个月后，采用Morris水迷宫实验评价大鼠的空间学习记忆能力。组织学研究用于海马的病理学分析。用生化和免疫荧光法检测MDA和GSH的变化及8-OHdG标记的DNA氧化损伤。在我们的研究结果中，照射组表现出显着的行为表现的变化，也表现出松散和水肿排列的病理特征。全脑MDA、GSH和8-OHdG含量均升高。此外，还可以通过免疫荧光法在海马中检测到8-OHdG表达的增加。我们的研究结果揭示了辐射诱导的氧化应激和行为缺陷之间的联系。这可能暗示了载人航天飞行中脑组织保护和风险评估的潜在机制。
The rapid growth of manned space flight results in more concerns about health risks and an urgent need for health assessment for space travel. The cosmic environment is complicated and full of radiation. Because of their strong biological effects, heavy ions such as56Fe ions are considered to be an important component of these lethal galactic rays. Due to the importance of brain function to astronauts, we explored the long-term effects and potential mechanisms of56Fe ion radiation on mice brains containing the hippocampus. In our study, radiation doses were carried out with 0.5 Gy, 1 Gy or 2 Gy. One month after whole-body56Fe ion exposure, the Morris water maze test was performed to assess the ability of spatial learning and memory. A histological study was used for pathology analysis of the hippocampus. Alteration of oxidative stress was reflected by MDA and GSH and oxidative DNA damage marked by 8-OHdG was detected by biochemical and immunofluorescence methods. In our results, irradiated groups exhibited significant changes in behavioral performance and also showed loose and edematous arrangement in the pathological characteristics. Furthermore, whole brain levels of MDA, GSH and 8-OHdG increased in the irradiated groups. In addition, increased expression of 8-OHdG can also be detected by immunofluorescence in the hippocampus. Our findings revealed a linkage between radiation-induced oxidative stress and behavioral deficits. This may suggest an underlying mechanism of brain tissue protection and risk assessment in manned space flight.