Combined effects of ethanol and L-menthol on hairless rat stratum corneum investigated by synchrotron X-ray diffraction.
Combined effects of ethanol and L-menthol on hairless rat stratum corneum investigated by synchrotron X-ray diffraction.
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DOI：
10.1016/j.jconrel.2006.08.005
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发表时间：
2006-10
期刊：
影响因子：
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通讯作者：
Y. Obata;I. Hatta;N. Ohta;N. Kunizawa;N. Yagi;K. Takayama
中科院分区：
文献类型：
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作者：
Y. Obata;I. Hatta;N. Ohta;N. Kunizawa;N. Yagi;K. Takayama
用同步辐射X射线衍射法研究了乙醇和薄荷醇对无毛大鼠角质层脂质结构的影响。在来源于软角蛋白的宽峰上观察到两个尖锐的衍射峰（S=2.40和S=2.67，分别对应于0.417 nm和0.374 nm的间距）。为了帮助理解用乙醇和l-薄荷醇处理的效果，将脂烃链堆积指数（RH/O）的丰度比定义为RH/O=（S=2.40 nm−1处的峰面积）/（S=2.67 nm−1处的峰面积）。当将乙醇施加到角质层时，衍射峰的强度略有下降。观察到的RH/O值不受乙醇浓度在0-40%（w/w）范围内变化的影响。甚至当用乙醇（40%，w/w）处理延长至8h时，RH/O值也没有变化。这些结果表明，角质层中的脂质排列不受乙醇的影响。另一方面，角质层暴露于2%（w/w）的l-薄荷醇引起RH/O值显着降低。结果表明，l-薄荷醇通过角质层分散，主要侵入六方烃链堆积，并破坏这些结构的规则组织。
Synchrotron X-ray diffraction was employed to evaluate the effect of ethanol and l-menthol on lipid arrangements in the stratum corneum of hairless rats. Two sharp diffractions (S=2.40 and S=2.67, corresponding to spacing of 0.417 nm and 0.374 nm respectively) were observed on the broad hump peak derived from soft keratin. To assist in understanding the effects of treatment with ethanol and l-menthol, an abundance ration of lipid hydrocarbon chain packing index (RH/O) was defined as RH/O=(Peak area at S=2.40 nm−1)/(Peak area at S=2.67 nm−1). When ethanol was applied to the stratum corneum the intensities of diffraction peaks declined slightly. The RH/Ovalues observed were not affected by variations in ethanol concentrations in the range 0–40% (w/w). The RH/Ovalues did not change even when treatment with ethanol (40%, w/w) was extended to 8 h. These results suggested that lipid arrangements in the stratum corneum were not affected by ethanol. On the other hand, exposure of the stratum corneum to 2% (w/w) l-menthol caused a significant decrease in RH/Ovalue. It was shown that l-menthol was dispersed through the stratum corneum, intruded mainly into hexagonal hydrocarbon chain packing, and disrupted the regular organization of these structures.