Genetic interactions at the FLA10 locus: suppressors and synthetic phenotypes that affect the cell cycle and flagellar function in Chlamydomonas reinhardtii.
Genetic interactions at the FLA10 locus: suppressors and synthetic phenotypes that affect the cell cycle and flagellar function in Chlamydomonas reinhardtii.
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作者：
F. G. Lux;S. Dutcher
通过分离莱茵衣藻FLA 10位点温度敏感性鞭毛组装突变的抑制子，我们已经确定了其他六个参与鞭毛组装的基因。在这些抑制基因座的突变，称为SUF 1-SUF 6，显示等位基因特异性相对于fla 10突变等位基因，他们抑制。另外一个突变apm 1 -122，赋予抗植物除草剂amiprostin-methyl和oryzalin，也被发现与突变在FLA 10基因座。apm 1 -122突变与三个fla 10突变等位基因的组合导致合成的冷敏感性细胞分裂缺陷，并与另外的假野生型fla 10等位基因的组合产生合成的温度敏感性鞭毛运动表型。基于这些基因座的遗传相互作用，我们提出FLA 10基因产物与鞭毛器的多个组件相互作用，并在鞭毛组装和细胞周期中发挥作用。
Through the isolation of suppressors of temperature-sensitive flagellar assembly mutations at the FLA10 locus of Chlamydomonas reinhardtii, we have identified six other genes involved in flagellar assembly. Mutations at these suppressor loci, termed SUF1-SUF6, display allele specificity with respect to which fla10- mutant alleles they suppress. An additional mutation, apm1-122, which confers resistance to the plant herbicides amiprophos-methyl and oryzalin, was also found to interact with mutations at the FLA10 locus. The apm1-122 mutation in combination with three fla10- mutant alleles results in synthetic cold-sensitive cell division defects, and in combination with an additional pseudo-wild-type fla10- allele yields a synthetic temperature-sensitive flagellar motility phenotype. Based upon the genetic interactions of these loci, we propose that the FLA10 gene product interacts with multiple components of the flagellar apparatus and plays a role both in flagellar assembly and in the cell cycle.