Studying the Dynamics of Flagella in Multicellular Communities of Escherichia coli by Using Biarsenical Dyes
Studying the Dynamics of Flagella in Multicellular Communities of Escherichia coli by Using Biarsenical Dyes
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DOI：
10.1128/aem.02153-09
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发表时间：
2010-02-01
影响因子：
4.4
通讯作者：
Weibel, Douglas B.
中科院分区：
文献类型：
--
作者：
Copeland, Matthew F.;Flickinger, Shane T.;Weibel, Douglas B.
本文介绍了一种新的方法，标记完整的鞭毛使用双砷染料FlAsH和ReAsH和成像的空间和时间动态的活大肠杆菌细胞在成群的细菌群落，通过使用落射荧光显微镜。使用这种方法，我们观察到（i）群集细胞上的鞭毛束在与相邻细胞频繁碰撞期间保持粘着，（ii）在殖民地前缘的非运动群集细胞上的鞭毛向未定殖琼脂表面的方向突出，并在从殖民地向外延伸的薄层流体中积极旋转，以及（iii）鞭毛与紧密靠近的其它群体细胞的鞭毛形成瞬时相互作用。这种方法为观察与生态学，工业和生物医学相关的社区中的细胞动态打开了一扇窗户。
This paper describes a new approach for labeling intact flagella using the biarsenical dyes FlAsH and ReAsH and imaging their spatial and temporal dynamics on live Escherichia coli cells in swarming communities of bacteria by using epifluorescence microscopy. Using this approach, we observed that (i) bundles of flagella on swarmer cells remain cohesive during frequent collisions with neighboring cells, (ii) flagella on nonmotile swarmer cells at the leading edge of the colony protrude in the direction of the uncolonized agar surface and are actively rotated in a thin layer of fluid that extends outward from the colony, and (iii) flagella form transient interactions with the flagella of other swarmer cells that are in close proximity. This approach opens a window for observing the dynamics of cells in communities that are relevant to ecology, industry, and biomedicine.