ATOMIC-FORCE MICROSCOPY PRODUCES FAITHFUL HIGH-RESOLUTION IMAGES OF PROTEIN SURFACES IN AN AQUEOUS ENVIRONMENT
ATOMIC-FORCE MICROSCOPY PRODUCES FAITHFUL HIGH-RESOLUTION IMAGES OF PROTEIN SURFACES IN AN AQUEOUS ENVIRONMENT
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DOI：
10.1073/pnas.91.3.836
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发表时间：
1994-02-01
影响因子：
11.1
通讯作者：
ENGEL, A
中科院分区：
文献类型：
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作者：
KARRASCH, S;HEGERL, R;ENGEL, A
原子力显微镜有可能监测生物系统在其自然环境中的结构变化。为了在分子水平上将它们与生物功能相关联，需要高的横向和纵向分辨率。在这里，我们表明，原子力显微镜是能够成像的六方包装的耐辐射球菌在缓冲溶液中的中间层的表面与1 nm的横向分辨率和0.1 nm的垂直分辨率。平均而言，这些形貌与电子显微镜测定的形貌不同，
The atomic force microscope has the potential to monitor structural changes of a biological system in its native environment. To correlate them with the biological function at a molecular level, high lateral and vertical resolution are required. Here we demonstrate that the atomic force microscope is capable of imaging the surface of the hexagonally packed intermediate layer of Deinococcus radiodurans in buffer solution with a lateral resolution of 1 nm and a vertical resolution of 0.1 nm. On average, these topographs differ from those determined by electron microscopy by