Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes.
Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes.
复制标题
新生儿和成人心肌细胞中外源 SERCA 基因表达对柯萨奇腺病毒受体水平的依赖性。
DOI：
10.1016/s0003-9861(03)00258-3
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发表时间：
2003
影响因子：
3.9
通讯作者：
Inesi,Giuseppe
中科院分区：
文献类型：
--
作者：
Sumbilla,Carlota;Ma,Hailun;Seth,Malini;Inesi,Giuseppe
我们证明，在原代培养的新生大鼠心肌细胞中，腺病毒辅助的外源性Ca~(2+)-ATPase(SERCA)和报告基因(EGFP)的表达效率远远高于成年大鼠心肌细胞的原代培养。在这方面，新生心肌细胞的行为类似于已建立的COS-1细胞系。这种差异与影响细胞穿透的柯萨奇腺病毒受体(CAR)水平和外源基因的表达水平有关，并解释了携带SERCA基因的腺病毒载体暴露后观察到的后果的变化。对这些差异的认识在新生儿和成人心肌细胞外源基因表达的互补研究中应该是非常有利的。在评估外源性SERCA基因传递到心肌的功能益处与可能的毒性效应的最佳比率的条件下，它也应该是有利的。
We demonstrate that the efficiency of adenovirus-assisted exogenous Ca2+ATPase (SERCA) and reporter (EGFP) gene expression is much higher in primary cultures of myocytes from neonatal rat hearts, than in primary cultures of myocytes from adult rat hearts. In this respect, the neonatal myocytes behave similarly to the established COS-1 cell line. This difference is related to the level of coxsackie adenovirus receptor (CAR) that affects cell penetration and expression level of exogenous genes, and explains variations in the observed consequences of exposure to adenovirus vector carrying SERCA cDNA. Awareness of these differences should be highly advantageous in complementary studies of exogenous gene expression in neonatal and adult myocytes. It should also be advantageous in evaluating conditions yielding optimal ratios of functional benefits over possible toxic effects upon exogenous SERCA gene delivery to cardiac muscle.